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|Purity Confirmation||> 90% by SDS-PAGE & visualized by silver stain|
|Molecular Weight||15.0 - 20.0 kDa|
|N Terminal Sequence||SIDNEWRKTQ AND NEWRKTQCMP|
|Biological Activity||The biological activity was determined (i) by the ability to induce VEGFR-3/FLT-4 receptor phosphorylation in PAEC/VEGFR-3 cells and (ii) the VEGF-C-induced proliferation of primary human dermal lymphatic endothelial cells (HDLEC).|
|Buffer||50 mM acetic acid|
|Reconstitution||The lyophilized VEGF-C is soluble in water and most aqueous buffers. The lyophilized VEGF-C should be reconstituted in PBS or medium to a concentration not lower than 50 µg/ml.|
|Stability and Storage||Lyophilized samples are stable for greater than six months at -20°C to -70°C. Reconstituted VEGF-C should be stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles!|
|Synonyms||vascular endothelial growth factor C; Vegfc|
|Description||VEGF-C, also known as Vascular Endothelial Growth Factor Related Protein (VRP), is a recently discovered VEGF growth factor family member that is most closely related to VEGF-D. The rat VEGF C cDNA encodes a pre-pro-protein of 416 amino acids residues. It is almost identical to the mouse VEGF-C protein. Similar to VEGF-D, VEGF-C has a VEGF homology domain spanning the middle third of the precursor molecule and long N- and C-terminal extensions. In adults, VEGF-C is highly expressed in heart, placenta, ovary and small intestine. Recombinant rat VEGF-C, lacking the N- and C-terminal extensions and containing only the middle VEGF homology domain, forms primarily non-covalently linked dimers. This protein is a ligand for both VEGFR-2/KDR and VEGFR-3/FLT-4. Since VEGFR-3 is strongly expressed in lymphatic endothelial cells, it has been postulated that VEGF-C is involved in the regulation of the growth and/or differentiation of lymphatic endothelium. Although recombinant rat VEGF-C is also a mitogen for vascular endothelial cells, it is much less potent than VEGF-A. The recombinant rat VEGF-C contains 127 amino acids residues and was fused to a His-tag (6x His) at the C-terminal end. As a result of glycosylation VEGF-C migrates as an 15-20 kDa protein in SDS-PAGE under reducing conditions.|
- Vascular Endothelial Growth Factor Receptor 3 Controls Neural Stem Cell Activation in Mice and Humans. J. Han et al., Cell Rep. 2015 Feb 24; 10(7): 1158–1172.
- VEGF-C improves regeneration and lymphatic reconnection of transplanted autologous lymph node fragments: An animal model for secondary lymphedema treatment. L. Schindewolffs et al., Immun Inflamm Dis. 2014 Nov; 2(3): 152–161.
- The Indolinone MAZ51 Induces Cell Rounding and G2/M Cell Cycle Arrest in Glioma Cells without the Inhibition of VEGFR-3 Phosphorylation: Involvement of the RhoA and Akt/GSK3β Signaling Pathways. Joo-Hee Park et al., PLoS One. 2014; 9(9): e109055.
- A Global Transcriptome Analysis Reveals Molecular Hallmarks of Neural Stem Cell Death, Survival, and Differentiation in Response to Partial FGF-2 and EGF Deprivation. V. Nieto-Estévez et al., PLoS One. 2013; 8(1): e53594.
- Vascular endothelial growth factor receptor 3 directly regulates murine neurogenesis. C.-F. Calvo et al., Genes Dev. 2011 Apr 15; 25(8): 831–844.
- Effects of VEGFR-3 phosphorylation inhibitor on lymph node metastasis in an orthotopic diffuse-type gastric carcinoma model. M. Yashiro et al., Br J Cancer. 2009 Oct 6; 101(7): 1100–1106.
- VEGF-C is a trophic factor for neural progenitors in the vertebrate embryonic brain. B. Le Bras et al., Nat Neurosci. 2006 Mar;9(3):340-8.
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