human Dkk-2

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Cat-Nr.100-436
Size10 µg
Price170 €
SourceCHO cells
Formulationlyophilized
Purity Confirmation> 98% by SDS-PAGE & HPLC analyses
Length [aa]234
Molecular Weight31-36 kDa
Endotoxin Levels< 0.1 ng/µg of protein (<1EU/µg)
Biological ActivityDetermined by its ability to inhibit alkaline phosphatase activity in differentiating MC3T3 E1 cells. The expected ED50 for this effect is 0.5– 1.0 µg/ml.
Species ReactivityHuman
SynonymsDickkopf-related protein-2, Dickkopf-2
DescriptionThe dickkopf (DKK)-related protein family is comprised of four central members, DKK-1 - 4, along with the distantly-related DKK family member DKK-11 (Soggy), which is thought to be a descendent of an ancestral DKK-3 precursor due to its unique sequence homology to DKK-3 and no other DKK family member. DKK family members, with the exception of the divergent Soggy, share two conserved cysteine-rich domains and show very little sequence similarity outside of these domains. Playing an important regulatory role in vertebrate development through localized inhibition of Wnt-regulated processes, including anterior-posterior axial patterning, limb development, somitogenesis, and eye formation, DKKs have also been implicated post-developmentally in bone formation, bone disease, cancer, and neurodegenerative diseases. DKK proteins typically play an important regulatory role in the Wnt/β-catenin signaling pathway by forming inhibitory complexes with LDL receptor-related proteins 5 and 6 (LRP5 and LRP6), which are essential components of the Wnt/β-catenin signaling system. LRP5 and LRP6 are single-pass transmembrane proteins that appear to act as co-receptors for Wnt ligands involved in the Wnt/β-catenin signaling cascade. DKK-2 has been shown to both inhibit and enhance canonical Wnt signaling; enhancing Wnt signaling through direct high-affinity binding of DKK-2 to LRP6 during LRP6 overexpression, while inhibiting Wnt signaling and promoting LRP6 internalization through the formation of a ternary complex between DKK-2, LRP6, and Kremen-2. Recombinant Human DKK-2 expressed in CHO cells is a glycoprotein that has a calculated molecular weight of 25.8 kDa and contains 234 amino acid residues. Due to glycosylation, human DKK-2 migrates at an apparent molecular weight of approximately 31-36 kDa by SDS-PAGE analysis under non-reducing conditions.
Protein SequenceSQIGSSRAKL NSIKSSLGGE TPGQAANRSA GMYQGLAFGG SKKGKNLGQA YPCSSDKECE VGRYCHSPHQ GSSACMVCRR KKKRCHRDGM CCPSTRCNNG ICIPVTESIL TPHIPALDGT RHRDRNHGHY SNHDLGWQNL GRPHTKMSHI KGHEGDPCLR SSDCIEGFCC ARHFWTKICK PVLHQGEVCT KQRKKGSHGL EIFQRCDCAK GLSCKVWKDA TYSSKARLHV CQ
Uniprot IDQ9UBU2
Protein RefSeqNP_055236
mRNA RefSeqNM_014421

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