Human Leptin N82K mutant, pegylated

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Cat-Nr.500-040
Size500 µg
Price440 €
SourceE. coli
LabelPEG
Formulationlyophilized
Purity Confirmation> 99.0% as determined by Gel filtration and SDS-PAGE gel.
Length [aa]146
Molecular Weight35.6 kDa
N Terminal SequenceAVPIQ
Endotoxin Levels< 0.1 ng/µg of protein (<1EU/µg)< 0.1 ng/µg of protein (<1EU/µg)
Biological ActivityRecombinant pegylated human leptin N82K mutant is less than 0.1% active active as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. This abolishment of activity results from drastically reduced affinity toward leptin receptor.
Species ReactivityHuman
ReconstitutionIt is recommended to reconstitute the lyophilized recombinant human pegylated leptin N82K mutant in sterile water or sterile 0.4% NaHCO3 adjusted to pH 8-9, not less than 100µg/ml, which can then be further diluted with other aqueous solutions.
SynonymsLep; ob; obese
DescriptionMono-pegylated (with 20 kDa PEG) recombinant human leptin N82K mutant is an one polypeptide chain containing 146 amino, an additional Ala at N-terminus and one molecule of PEG 20 kDa at its N-terminus, having an expected molecular mass of ~ 35.6 kDa as determined by MS. However due to enlarged hydrodymanic volume recombinant human pegylated leptin runs on the SGS-Page as 48 kDa protein and in gel-filtration on Superdex 200 as over 200 kDa protein. Recombinant human pegylated leptin N82K mutant's half-life in circulation after SC injection was over 20 hours. Recombinant human leptin N82K mutant was purified by proprietary chromatographic techniques according to Salomon et al (2006) Protein Expression and Purification 47, 128–136 and then pegylated.
Protein SequenceAVPIQKVQDDTKTLIKTIVTRINDISHTQSVSSKQKVTGLDFIPGLHPILTLSKMDQTLAVYQQILTSMPSRNVIQISNDLEKLRDLLHVLAFSKSCHLPWASGLETLDSLGGVLEASGYSTEVVALSRLQGSLQDMLWQLDLSPGC
Uniprot IDP41159
Protein RefSeqNP_000221.1
mRNA RefSeqNM_000230

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