Rat FGF acidic

Slide this table

Cat-Nr.R20-018S
Size10 µg
Price75 €
SourceE. coli
Formulationlyophilized
Purity Confirmation> 98% by SDS-PAGE & HPLC analyses
Length [aa]141
Molecular Weight15.9 kDa
Endotoxin Levels< 0.1 ng/µg of protein (<1EU/µg)
Biological ActivityDetermined by a cell proliferation assay using balb/c 3T3 cells.  The expected ED50 is ≤ 0.1 ng/ml, in the presence of 10 µg/ml heparin, corresponding to a specific activity of ≥ 1 x 107 units/mg.
Species ReactivityRat
Buffer10mM Sodium Phosphate, pH 7.2 + 150mM NaCl + 0.2% Chaps
ReconstitutionReconstitute in 5 mM Sodium Phosphate, pH 7.2 to a concentration of ≤ 0.5 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Stability and StorageThe lyophilized protein is stable at room temperature for 1 month and at 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C.
SynonymsFgf1; FGF-1; HBGF1; HBGF-1
DescriptionFGF-acidic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-acidic is a non-glycosylated heparin binding growth factor that is expressed in the brain, kidney, retina, smooth muscle cells, bone matrix, osteoblasts, astrocytes and endothelial cells. FGF-acidic has the ability to signal through all the FGF receptors. Recombinant rat FGF-acidic is a 15.9 kDa protein consisting of 141 amino acid residues.
Protein SequenceMFNLPLGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SAGEVYIKGT ETGQYLAMDT EGLLYGSQTP NEECLFLERL EENHYNTYTS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D
Uniprot IDP61149
Protein RefSeqNP_036978.1
mRNA RefSeqNM_012846.1

All prices plus VAT + possible delivery charges