Pufferfish Leptin

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Size100 µg
Price210 €
SourceE. coli
Purity Confirmation> 98.0% as determined by Gel filtration and SDS-PAGE gel.
Length [aa]134
Molecular Weight15.3 kDa (momnomer)
N Terminal SequenceALPGA
Endotoxin Levels< 0.1 ng/µg of protein (<1EU/µg)
Biological ActivityRecombinant fish leptin is capable of inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. The affinity of human leptin receptors is considerably lower campared to mammalian leptins.
Species ReactivityPufferfish
ReconstitutionIt is recommended to reconstitute the lyophilized recombinant fish leptin in sterile water or sterile 0.4% NaHCO3 adjusted to pH 8-9, not less than 100µg/ml, which can then be further diluted with other aqueous solutions.
SynonymsLep; ob; obese
DescriptionThe first biologically active recombinant fish leptin was prepared according to the sequence published by Kurokawa et al. (2005)Peptides 26, 745-750 in two forms: monomer and covalent dimer. MS analysis revealed molecular masses of 15,291 and 30,585 Da, close to the theoretical values of 15,270 and 30,540 Da. CD spectra revealed high similarity to mammalian leptins. Other details of its preparation will be soon published by Yacobovitz et al , General and Comparative Endocrinology (2008) 156(1):83-90.
Uniprot IDQ588G0
Protein RefSeqNP_001027897.1
mRNA RefSeqNM_001032725.1

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