Mouse IL-22 pegylated

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Cat-Nr.500-029
Size50 µg
Price950 €
SourceE. coli
LabelPEG
Formulationlyophilized
Purity Confirmation> 98.0% as determined by Gel filtration and SDS-PAGE gel.
Length [aa]147
Molecular Weight36.0 kDa (monomer)
N Terminal SequenceALPVN
Endotoxin Levels< 0.1 ng/µg of protein (<1EU/µg)
Biological ActivityRecombinant murine IL-22 is biologically active when compared to standard using STAT3 phosphorylation assay in HepG cells. Its acrtivity in vitro is ~ 10% compared to the non-pegylated mIL22.
Species ReactivityMouse
ReconstitutionIt is recommended to reconstitute the lyophilized pegylated mouse IL22 in sterile water or sterile 0.4% NaHCO3 adjusted to pH 8-9, not less than 100µg/ml, which can then be further diluted with other aqueous solutions.
SynonymsIL22; IL-22; Iltif; IL-22a; ILTIFa
DescriptionMono-pegylated (with 20 kDa PEG)recombinant mouse interleukin 22 is one polypeptide chain containing 147 amino, an additional Ala at N-terminus and one molecule of PEG 20 kDa at its N-terminus, having an expected molecular mass of ~ 36 kDa as determined by MS. However due to enlarged hydrodymanic volume it runs on the SDS-PAGE as 50 kDa protein and in gel-filtration on Superdex 200 as over 200 kDa protein. For more details see L. Niv-Spector et al. Protein Eng Des Sel. (PEDS) 25:397-404 (2012).
Protein SequenceALPVNTRCKL EVSNFQQPYI VNRTFMLAKE ASLADNNTDV RLIGEKLFRG VSAKDQCYLM KQVLNFTLED VLLPQSDRFQ PYMQEVVPFL TKLSNQLSSC HISGDDQNIQ KNVRRLKETV KKLGESGEIK AIGELDLLFM SLRNACV
Uniprot IDQ9JJY9
Protein RefSeqNP_058667.1
mRNA RefSeqNM_016971

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