Human IL-35

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Cat-Nr.100-164S
Size2 µg
Price95 €
SourceHEK 293 cells
Formulationlyophilized
Purity Confirmation≥ 90% by SDS-PAGE gel and HPLC analyses.
Length [aa]406
Molecular Weight45.8 kDa
Endotoxin Levels< 0.1 ng/µg of protein (<1EU/µg)
Biological ActivityMeasured by its ability to inhibit proliferative activity of PBMCs.
Species ReactivityHuman, Mouse
SynonymsInterleukin-35
DescriptionInterleukin-35 is a glycosylated, heterodimeric protein consisting of the p35 subunit from IL-12 (IL-12α) and the β subunit from IL-27 (EBI3). IL-35 can be expressed by regulatory T-cells (Tregs), macrophages, and certain trophoblast and dendritic cells. It is induced in response to inflammation, and generally acts as an inflammation suppressor. IL-35 suppresses inflammation by exerting multiple activities, including the induction of regulatory T-cells and the suppression of Th17 cells. Recombinant Human IL-35 produced from HEK293 cells is a glycosylated protein, primarily heterodimeric, that migrates as a diffuse band centered at an apparent molecular weight of about 60 kDa by SDS-PAGE analysis under non-reducing conditions. Recombinant Human IL-35 expressed in HEK293 cells contains 406 amino acid residues and has a calculated molecular weight of 45.8 kDa.
Protein Sequenceα Subunit: RNLPVATPDP GMFPCLHHSQ NLLRAVSNML QKARQTLEFY PCTSEEIDHE DITKDKTSTV EACLPLELTK NESCLNSRET SFITNGSCLA SRKTSFMMAL CLSSIYEDLK MYQVEFKTMN AKLLMDPKRQ IFLDQNMLAV IDELMQALNF NSETVPQKSS LEEPDFYKTK IKLCILLHAF RIRAVTIDRV MSYLNAS β Subunit: RKGPPAALTL PRVQCRASRY PIAVDCSWTL PPAPNSTSPV SFIATYRLGM AARGHSWPCL QQTPTSTSCT ITDVQLFSMA PYVLNVTAVH PWGSSSSFVP FITEHIIKPD PPEGVRLSPL AERQLQVQWE PPGSWPFPEI FSLKYWIRYK RQGAARFHRV GPIEATSFIL RAVRPRARYY VQVAAQDLTD YGELSDWSLP ATATMSLGK
Uniprot IDAlpha: P29459 Beta: Q14213
Protein RefSeqAlpha: NP_001384921 Beta: NP_005746
mRNA RefSeqAlpha: NM_001397992 Beta: NM_005755

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