Human FGF-2 (basic) heat stable, 154 aa

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Cat-Nr.100-450S-HS
Size10 µg
Price155 €
SourceE. coli
Formulationlyophilized
Purity Confirmation≥ 95% by SDS-PAGE gel and HPLC analyses.
Length [aa]154
Molecular Weight17.2 kDa
Endotoxin Levels< 0.1 ng/µg of protein (< 1EU/µg)
Biological ActivityDetermined by a cell proliferation assay using Balb/c 3T3 cells. The expected ED50 is 0.05-0.25 ng/ml.
Species ReactivityHuman
Buffer5mM Tris, pH 7.6 + 150mM NaCl
ReconstitutionCentrifuge vial prior to opening. Reconstitute in 5mM Tris, pH 7.6, to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Stability and StorageThe lyophilized protein is stable at room temperature for 1 month and at 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 12 months at -20°C to -80°C.
SynonymsFibroblast Growth Factor-basic, FGF-2, HBGF-2, Prostatropin
DescriptionFGF-basic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-basic exerts proliferative activity towards a wide range of cell types and consequently is used as a vital component in numerous cell culture media formulations, including stem cell media. FGF-basic loses its activity over time in solution at physiological temperatures and needs to be resupplemented periodically to maintain its performance. As a result of selected amino acid substitutions, Heat Stable FGF-basic retains full biological activity and also maintains full activity over extended periods of time in solution at 37°C. These properties make Heat Stable FGF-basic a superior choice for use in cell culture media applications. Heat Stable Recombinant Human FGF-basic is a 17.2 kDa protein consisting of 154 amino acids.
Protein SequenceAAGSITTLPA LPEDGGSGAF PPGHFKDPKL LYCKNGGFFL RIHPDGRVDG TRDKSDPFIK LQLQAEERGV VSIKGVCANR YLAMKEDGRL YAIKNVTDEC FFFERLEENN YNTYRSRKYP SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS
Uniprot IDP09038
Protein RefSeqNP_001997.5
mRNA RefSeqNM_002006.4

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