Human M-CSF

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Cat-Nr.100-064
Size10 µg
Price170 €
SourceE. coli
Formulationlyophilized
Purity Confirmation> 98% by SDS-PAGE
Length [aa]159
Molecular Weight36.8 kDa
Biological ActivityThe ED50, calculated by the dose-dependent stimulation of the proliferation of murine M-NFS-60 indicator cells was found to be < 1.0ng/ml.
Species ReactivityMonkey, Mouse, Rat, Human
BufferPBS
SynonymsCSF1; MCSF; CSF-1
DescriptionGranulocyte/Macrophage Colony-Stimulating Factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy. Macrophage Colony Stimulating Factor Human Recombinant produced in E. coli is a disulfide linked homodimer, non-glycosylated, polypeptide chain containing 2 x 159 amino acids and having a total molecular mass of 36.8 KD. M-CSF is purified by proprietary chromatographic techniques.
Protein SequenceMEEVSEYCSH MIGSGHLQSL QRLIDSQMET SCQITFEFVD QEQLKDPVCY LKKAFLLVQD IMEDTMRFRD NTPNAIAIVQ LQELSLRLKS CFTKDYEEHD KACVRTFYET PLQLLEKVKN VFNETKNLLD KDWNIFSKNC NNSFAECSSQ GHERQSEGS
Uniprot IDP09603
Protein RefSeqNP_000748.3
mRNA RefSeqNM_000757

Reference

  1. Lymphocyte-specific protein 1 regulates mechanosensory oscillation of podosomes and actin isoform-based actomyosin symmetry breaking. Cervero P. et al., Nat Commun. 2018 Feb 6;9(1):515.
  2. TGF-β1-dependent L1CAM expression has an essential role in macrophage-induced apoptosis resistance and cell migration of human intestinal epithelial cells. Schäfer H et al., Oncogene. 2013 Jan 10;32(2):180-9.
  3. Inflammatory Macrophages Induce Nrf2 Transcription Factor-dependent Proteasome Activity in Colonic NCM460 Cells and Thereby Confer Anti-apoptotic Protection. S. Sebens et al., J Biol Chem. 2011 Nov 25; 286(47): 40911–40921.

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