Human TNF-alpha

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Cat-Nr.300-028
Size50 µg
Price135 €
SourceE. coli
Formulationlyophilized
Purity Confirmation> 95% by SDS-PAGE and RP-HPLC analysis
Length [aa]158
Molecular Weight17.5 kDa
Biological ActivityThe specific activity is > 5.0×107 IU/mg as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D.
Species ReactivityHuman
SynonymsTNF; DIF; TNFA; TNFSF2; TNF-alpha
DescriptionTumor necrosis factor is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is mainly secreted by macrophages. TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis and viral replication, TNF is also involved in lipid metabolism, and coagulation. TNF's primary role is in the regulation of immune cells. Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases- autoimmune diseases, insulin resistance, and cancer. Recombinant human Tumor Necrosis Factor-α produced in E.coli is a single, non-glycosylated, polypeptide chain containing 158 amino acids and having a molecular mass of 17483.77 Dalton.
Protein SequenceMVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA E AKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL
Uniprot IDP01375
Protein RefSeqNP_000585.2
mRNA RefSeqNM_000594

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