Human IL-12p70

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Cat-Nr.100-027
Size10 µg
Price170 €
SourceCHO cells
Formulationlyophilized
Purity Confirmation> 98% by SDS-PAGE & HPLC analyses
Length [aa]503
Molecular Weight65-70 kDa
Endotoxin Levels< 0.1 ng/µg of protein (<1EU/µg)
Biological ActivityDetermined by its ability to increase IFN-γ production by anti-TCR mAb-stimulated PBMCs.
Species ReactivityHuman
SynonymsInterleukin-12, NKSF, CTL Maturation Factor (TCMF), Cytotoxic Lymphocyte Maturation Factor (CLMF), TSF
DescriptionIL-12 is a potent regulator of cell-mediated immune responses and it induces IFN-γ production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes and connective tissue-type mast cells. Among its biological activities, IL-12 promotes the growth and activity of activated NK, CD4+ and CD8+ cells, and induces the development of IFN-γ-producing Th1 cells. Recombinant Human IL-12 p70, derived from HEK293 cells, is a heterodimeric glycoprotein consisting of disulfide-linked p35 and p40 subunits (503 total amino acid residues). The calculated molecular weight of Human IL-12 p70 is 57.2 kDa; however, due to glycosylation, it migrates at an apparent molecular weight of 65-70 kDa based on SDS-PAGE gel, under non-reducing conditions.
Protein Sequencep35 Subunit: RNLPVATPDP GMFPCLHHSQ NLLRAVSNML QKARQTLEFY PCTSEEIDHE DITKDKTSTV EACLPLELTK NESCLNSRET SFITNGSCLA SRKTSFMMAL CLSSIYEDLK MYQVEFKTMN AKLLMDPKRQ IFLDQNMLAV IDELMQALNF NSETVPQKSS LEEPDFYKTK IKLCILLHAF RIRAVTIDRV MSYLNAS p40 Subunit: IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCS
Uniprot IDP29460
Protein RefSeqNP_002178.2
mRNA RefSeqNM_002187.2

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