Human PDGF-BB

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Cat-Nr.200-056
Size20 µg
Price175 €
SourceE. coli
Formulationlyophilized
Purity Confirmation> 95% by SDS-PAGE & visualized by silver stain
Length [aa]109
Molecular Weight24.3 kDa
Endotoxin Levels< 0.1ng per ug of PDGF-BB
Biological ActivityThe biological activity was determined by the induction of proliferation in NHDF cells (Normal Human Dermal Fibroblasts).
Species ReactivityHuman
Buffer50mM acetic acid
ReconstitutionCentrifuge vial prior to opening. The lyophilized PDGF-BB should be reconstituted in 50mM acetic acid to a concentration not lower than 100μg/ml. For long term storage of reconstituted protein addition of carrier protein (e.g. BSA or HSA; 0.1%) is recommended.
Stability and StorageThe lyophilized PDGF-BB is stable for a few weeks at room temperature, but best stored at -20 °C. Reconstituted PDGF-BB is best stored at -20 °C to -70 °C.
SynonymsPDGF-2; Platelet-derived growth factor B chain; Platelet-derived growth factor beta polypeptide; Proto-oncogene c-Sis; INN=Becaplermin
DescriptionPDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs; PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet alpha-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-alpha and PDGFR-beta. PDGFR-alpha is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-beta interacts with only PDGF-BB and PDGF-AB. Recombinant human PDGF-BB is a 24.3 kDa disulfide-linked homodimer of two B chains (218 total amino acids).
Protein SequenceSLGSLTIAEPAMIAECKTRTEVFEISRRLIDRTNANFLVWPPCVEVQRCSGCCNNRNVQCRPTQVQLRPVQVRKIEIVRKKPIFKKATVTLEDHLACKCETVAAARPVT
Uniprot IDP01127
Protein RefSeqNP_002599.1
mRNA RefSeqNM_002608.2

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