Pleurotus ostreatus Ostreolysin (Cytolysin)

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Size100 µg
Price310 €
SourceE. coli
Purity Confirmation> 98.0% as determined by Gel filtration and SDS-PAGE gel.
Length [aa]137
Molecular Weight14.9 kDa
N Terminal SequenceAYAQWV
Endotoxin Levels< 0.1 ng/µg of protein (<1EU/µg)
Biological ActivityOstreolysin has potent anti-carcinogenic activity in several colon cancer cell lines.
Species ReactivityOyster mushroom
ReconstitutionIt is recommended to reconstitute the lyophilized ostreolysin in sterile 0.4% NaHCO3 adjusted, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
SynonymsOstreolysin A6
DescriptionOstreolysin, is a cytosolic protein of 15 kDa pore-forming protein from the edible oyster mushroom (Pleurotus ostreatus), is lytic to membranes containing both cholesterol and sphingomyelin. Its cytotoxicity to Chinese hamster ovary cells correlates with their cholesterol contents and with the occurrence of ostreolysin in the cells detergent resistant membranes. Moreover, ostreolysin binds to supported monolayers and efficiently permeabilizes sonicated lipid vesicles, only if cholesterol is combined with either sphingomyelin or dipalmitoyl-phosphatidylcholine. Addition of mono- or di-unsaturated phosphatidylcholine to the cholesterol/sphingomyelin vesicles dramatically reduces the ostreolysin's activity. It appears that the protein recognizes specifically a cholesterol-rich lipid phase, probably the liquid-ordered phase. Ostreolysin was purified by combination of ion-exchange and size-exclusion chromatography.
Uniprot IDP83467
Protein RefSeqAGH25589.1
mRNA RefSeqKC012711.1

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