Human LIF

Slide this table

Cat-Nr.200-008
Size50 µg
Price290 €
SourceE. coli
Formulationlyophilized
Purity Confirmation> 98% by SDS-PAGE & visualized by silver stain
Length [aa]180
Molecular Weight19.7 kDa
N Terminal SequenceSPLPIT
Endotoxin Levels< 0.1ng per µg (Ieu/µg) of rh LIF
Biological ActivityThe ED50 as determined by the M1 cell differentiation assay is in the range of 0.1 - 0.5 ng/ml.
Species ReactivityHuman
Buffer50mM acetic acid
ReconstitutionThe lyophilized LIF should be reconstituted in water to a concentration not less than 100µg/ml. This solution can be diluted into other buffered solutions or stored at -20 °C for future use.
Stability and StorageLyophilized Leukemia Inhibitory Factor (LIF) although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution Leukemia Inhibitory Factor (LIF) should be stored at 4 °C between 2-7 days and for future use below -18 °C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
SynonymsLIF; CDF; DIA; HILDA; MLPLI
DescriptionLeukemia Inhibitory Factor also called LIF is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Human and mouse LIF exhibit a 78% identity in its amino acid sequence. Human LIF is as active on human cells as is it is on mouse cells, though mouse LIF is about 1000 fold less active on human cells, than human LIF. Recombinant human LIF produced in E. coli is a single, non-glycosylated, polypeptide chain containing 180 amino acids and having a molecular mass of 19.7 kDa.
Protein SequenceSPLPITPVNATCAIRHPCHNNLMNQIRSQLAQLNGSANALFILYYTAQGEPFPNNLDKLCGPNVTDFPPFHANGTEKAKLVELYRIVVYLGTSLGNITRDQKILNPSALSLHSKLNATADILRGLLSNVLCRLCSKYHVGHVDVTYGPDTSGKDVFQKKKLGCQLLGKYKQIIAVLAQAF
Uniprot IDP15018
Protein RefSeqNP_002300.1
mRNA RefSeqNM_002309.4

All prices plus VAT + possible delivery charges