Ovine Leptin antagonist (mutant L39A/D40A/F41A)

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Cat-Nr.500-065S
Size50 µg
Price175 €
SourceE. coli
Formulationlyophilized
Purity Confirmation> 98.0% as determined by Gel filtration and SDS-PAGE gel.
Length [aa]146
Molecular Weight16.0 kDa
N Terminal SequenceAVPIR
Endotoxin Levels< 0.1 ng/µg of protein (<1EU/µg)
Biological ActivityOvine recombinant leptin antagonist is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of ovine leptin receptor. It also inhibits various leptin effects in several in vitro bioassays.
Species ReactivityOvine
ReconstitutionIt is recommended to reconstitute the lyophilized recombinant leptin antagonist in sterile water or 0.4% NaHCO3 adjusted to pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
SynonymsLep; ob; obese
DescriptionRecombinant ovine leptin, one polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa, oLEP was mutated, resulting in L39A/D40A/F41A mutant was purified by proprietary chromatographic techniques. Preparation of recombinant leptin antagonists was recently published - Niv-Spector et al, Biochem. J 291;221-230 (2005).
Protein SequenceAVPIRKVQDDTKTLIKTIVTRINDISHTQSVSSKQRVTGAAAIPGLHPLLSLSKMDQTLAIYQQILASLPSRNVIQISNDLENLRDLLHLLAASKSCPLPQVRALESLESLGVVLEASLYSTEVVALSRLQGSLQDMLRQLDLSPG
Protein RefSeqXP_027824581.2
mRNA RefSeqXM_027968780.2

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