Mouse IL-28A

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Cat-Nr.M10-029
Size20 µg
Price170 €
SourceE. coli
Formulationlyophilized
Purity Confirmation> 95% by SDS-PAGE & HPLC analyses
Length [aa]174
Molecular Weight19.7kDa
Endotoxin Levels< 0.1 ng /mg of protein (<1EU/mg).
Biological ActivityThe ED50 as determined by an anti-viral assay using human HepG2 cells infected with encephalomyocarditis(EMC) virus is less than 3 ng/ml, corresponding to a specific activity of > 3.3 × 105 IU/mg.
Species ReactivityMouse
BufferPBS/5% Trehalose
ReconstitutionIt is recommended to reconstitute the lyophilized IL-28A in sterile water not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability and StorageLyophilized mouse IL-28A although stable at room temperature for 3 weeks, should be stored desiccated below -18°C.
SynonymsInterferon lambda-2, IFN-lambda-2, Interleukin-28A, IL-28A, Ifnl2, Il28a, EG330496.
DescriptionIL-28A is distantly related to type I interferons and the IL-10 family. Expression of IL-28A is induced by viral infection which interacts with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor, beta (IL10RB) and interleukin 28 receptor, alpha. IL-28A exhibits common features with type I IFNs such as antiviral activity, antiproliferative activity and in vivo antitumour activity. IL-28A acts similarly to IFNs, but is less effective generally and has activity in a more limited range of cell lines. IFN-ambda 1, IFN-lambda 2 and IFN-lambda 3 are closely positioned genes on human chromosome 19. IL-28A induces ELR(-) CXC chemokine mRNA in human peripheral blood mononuclear cells, in an IFN-gamma-independent manner. IL-28A is able to generate tolerogenic DCs, an activity that could thwart IFN-beta functions. IL-28A produced in response to viral infection, activates both monocytes and macrophages producing a restricted panel of cytokines and therefore is an important factor in activating innate immune responses at the site of viral infection.
Protein SequenceDPVPRATRLP VEAKDCHIAQ FKSLSPKELQ AFKKAKDAIE KRLLEKDMRC SSHLISRAWD LKQLQVQERP KALQAEVALT LKVWENMTDS ALATILGQPL HTLSHIHSQL QTCTQLQATA EPKPPSRRLS RWLHRLQEAQ SKETPGCLED SVTSNLFRLL TRDLKCVASG DQCV
Uniprot IDQ4VK74
Protein RefSeqNP_001019844.2
mRNA RefSeqNM_001024673.2

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