Human FGF-4

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Cat-Nr.300-130
Size5 µg
Price65 €
SourceE. coli
Formulationlyophilized
Purity Confirmation> 95% by SDS-PAGE & visualized by silver stain
Length [aa]177
Molecular Weight19.7 kDa
N Terminal SequenceMAPTAPNGTL
Biological ActivityThe biological activity was determined by the induction of proliferation in NHDF cells (Normal Human Dermal Fibroblasts).
Species ReactivityMouse, Rat, Human, Pig
BufferPBS
ReconstitutionWe recommend a quick spin followed by reconstitution in water to a concentration of 0.1-1.0mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4 °C for 1 week or -20 °C for future use.
Stability and StorageThe lyophilized protein is stable for a few weeks at room temperature, but best stored at -20 °C. Reconstituted FGF-4 should be stored in working aliquots at -20 °C. Avoid repeated freeze-thaw cycles.
SynonymsFGF4; HST; KFGF; HST-1; HSTF1; K-FGF; HBGF-4
DescriptionFGF-4 (fibroblast growth factor 4), also known as K-FGF (Kaposi’s sarcoma associated FGF), is a 25 kDa secreted, heparin binding member of the FGF family. The human FGF-4 cDNA encodes 206 amino acids (aa) with a 33 aa signal sequence and a 173 aa mature protein with an FGF homology domain that contains a heparin binding region near the C terminus. Mature human FGF-4 shares a high aa identity with mouse, rat, canine and bovine FGF-4, respectively. The expression of FGF-4 and its receptors, FGF-R1c, -R2c, -R3c and R4, is spatially and temporally regulated during embryonic development. Its expression in the mouse trophoblast inner cell mass promotes expression of FGF-R2, and is required for maintenance of the trophectoderm and primitive endoderm. FGF-4 is proposed to play a physiologically relevant role in human embryonic stem cell self-renewal. It promotes stem cell proliferation, but may also aid differentiation depending on context and concentration, and is often included in embryonic stem cell media in-vitro. FGF-4 is mitogenic for fibroblasts and endothelial cells in-vitro and has autocrine transforming potential. It is a potent angiogenesis promoter in-vivo and has been investigated as therapy for coronary artery disease.
Protein SequenceAPTAPNGTLEAELERRWESLVALSLARLPVAAQPKEAAVQSGAGDYLLGIKRLRRLYCNVGIGFHLQALPDGRIGGAHADTRDSLLELSPVERGVVSIFGVASRFFVAMSSKGKLYGSPFFTDECTFKEILLPNNYNAYESYKYPGMFIALSKNGKTKKGNRVSPTMKVTHFLP RL
Uniprot IDP08620
Protein RefSeqNP_001998.1
mRNA RefSeqNM_002007

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