Fish (carp) Growth Hormone

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Size10 µg
Price190 €
SourceE. coli
Purity Confirmation> 95.0% as determined by RP-HPLC, Gel filtration and SDS-PAGE Silver Stained gel.
Length [aa]188
Molecular Weight21.4 kDa
N Terminal SequenceSDNQRLF
Endotoxin Levels< 0.1 ng/µg of protein (<1EU/µg)
Biological ActivityRecombinant common carp growth hormone is biologically active in rat 3T3 F442A preadipocytes, though its activity is 15-fold lower compared to bovine GH, but it is equally potent in vivo in promoting carp growth (Fine et al.1993). Recombinant common carp growth hormone is capable to form a 1:2 complex with oGHR-ECD and induce proliferation of PFD-P1 cells stably transfected with rabbit growth hormone receptor, though its activity is 5-fold lower compared to bovine GH (A. Gertler, unpublished data).
ReconstitutionIt is recommended to reconstitute the lyophilized recombinant common carp growth hormone in sterile water or 0.4% NaHCO3 adjusted to pH 8-9, not less than 100µg/ml and not more than 3 mg/ml. Recombinant common carp growth hormone can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.
SynonymsSomatotropin, GH, GH-N, Growth Hormone 1, Pituitary growth hormone
DescriptionRecombinant common carp (Cyprinus carpio) growth hormone (caGH) produced in E.Coli (21.4 K) is a single, non-glycosylated, polypeptide chain containing 188 amino acids and having a molecular mass of 21404 Da. Recombinant common carp growth hormone is purified by chromatographic techniques, according to Fine et al. General and Comparative Endocrinology, 89,51-61 (1993).
Uniprot IDP10298
Protein RefSeqXP_018951738.1
mRNA RefSeqXM_019096193.2

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