Human R-Spondin-3

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Cat-Nr.100-427S
Size5 µg
Price75 €
SourceCHO cells
Formulationlyophilized
Purity Confirmation> 95% by SDS-PAGE & HPLC analyses
Length [aa]240
Molecular Weight26.9 kDa
Endotoxin Levels< 0.1 ng /mg of protein (<1EU/mg).
Biological ActivityR-Spondin-3 enhances BMP-2 mediated differentiation of MC3T3-E1 cells.
Species ReactivityHuman
ReconstitutionCentrifuge the vial prior to opening!
SynonymsPWTSR, THSD2, Roof plate-specific Spondin-3, RSPO3
DescriptionThe R-Spondin (Rspo) proteins belong to the Rspo family of Wnt modulators. Currently, the family consists of four structurally related secreted ligands (Rspo 1-4), all containing furin-like and thrombospondin structural domains. The Rspo proteins can interact with the Frizzled/LRP6 receptor complex in a manner that causes the stabilization and resulting accumulation of the intracellular signaling protein, β-catenin. This activity effectively activates and increases the subsequent nuclear signaling of β-catenin. R-Spondin can also bind to the previously discovered G-protein coupled receptors, LGR-4 and LGR-5. Rspo/β-catenin signaling can act as an inducer of the transformed phenotype, and can also regulate the proliferation and differentiation of certain stem cell populations.  Recombinant human R-Spondin-3 is a 26.9 kDa protein consisting of 240 amino acid residues.  Due to glycosylation, R-Spondin-3 migrates at an apparent molecular weight of approximately 37.0 kDa by SDS PAGE analysis under reducing conditions.
Protein SequenceMHPNVSQGCQ GGCATCSDYN GCLSCKPRLF FALERIGMKQ IGVCLSSCPS GYYGTRYPDI NKCTKCKADC DTCFNKNFCT KCKSGFYLHL GKCLDNCPEG LEANNHTMEC VSIVHCEVSE WNPWSPCTKK GKTCGFKRGT ETRVREIIQH PSAKGNLCPP TNETRKCTVQ RKKCQKGERG KKGRERKRKK PNKGESKEAI PDSKSLESSK EIPEQRENKQ QQKKRKVQDK QK
Uniprot IDQ9BXY4
Protein RefSeqNP_116173.2
mRNA RefSeqNM_032784.4

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