Human Leptin N82K mutant

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Size500 µg
Price230 €
SourceE. coli
Purity Confirmation> 98.0% as determined by Gel filtration and SDS-PAGE gel.
Length [aa]146
Molecular Weight16.0 kDa
N Terminal SequenceAVPIQ
Endotoxin Levels< 0.1 ng/µg of protein (<1EU/µg)
Biological ActivityRecombinant human leptin N82K mutant is less than 0.1% active active as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. This abolishment of activity results from drastically reduced affinity toward leptin receptor.
Species ReactivityHuman
ReconstitutionIt is recommended to reconstitute the lyophilized recombinant human leptin N82K mutant in sterile water or 0.4% NaHCO3 adjusted to pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
SynonymsLep; ob; obese
DescriptionRecombinant protein - human leptin N82K mutant was produced by site specific mutagenesis. It consists of one polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa., Human leptin mutant was purified by proprietary chromatographic techniques, see Niv-Spector et al. (2010) Mol Genet Metab. 100(2):193-7. It is devoid of biological activity and mey serve for control experiments.
Uniprot IDP41159
Protein RefSeqNP_000221.1
mRNA RefSeqNM_000230

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