Dog Leptin

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Cat-Nr.500-060
Size500 µg
Price510 €
SourceE. coli
Formulationlyophilized
Purity Confirmation> 98.0% as determined by Gel filtration and SDS-PAGE gel.
Length [aa]146
Molecular Weight16.0 kDa
N Terminal SequenceAVPIR
Endotoxin Levels< 0.1 ng/µg of protein (<1EU/µg)
Biological ActivityRecombinant dog leptin is fully biologically active as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor.
Species ReactivityDog
ReconstitutionIt is recommended to reconstitute the lyophilized recombinant dog leptin in sterile 0.4% NaHCO3 adjusted, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
SynonymsLep; ob; obese
DescriptionRecombinant dog leptin is an one polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa. Dog leptin was purified by proprietary chromatographic techniques, (Solomon, Charlier, Djiane and Gertler, unpublished results).
Protein SequenceAVPIRKVQDDTKTLIKTIVARINDISHTQSVSSKQRVAGLDFIPGLQPVLSLSRMDQTLAIYQQILNSLHSRNVVQISNDLENLRDLLHLLASSKSCPLPRARGLETFESLGGVLEASLYSTEVVALNRLQAALQDMLRRLDLSPGC
Uniprot IDO02720
Protein RefSeqNP_001003070.1
mRNA RefSeqNM_001003070.1

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