Human IL-22 receptor antagonist (Y51A), pegylated

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Size10 µg
Price310 €
SourceE. coli
Purity Confirmation> 98.0% as determined by Gel filtration and SDS-PAGE gel.
Length [aa]147
Molecular Weight36.0 kDa (monomer)
Endotoxin Levels< 0.1 ng/µg of protein (<1EU/µg)
Biological ActivityRecombiant human IL-22 Y51A mutant is biologically active, capable of full inhibition of mouse IL-22 induced STAT3 phosphorylation in HepG cells. Its inhibitory acrtivity in vitro is ~ 10-20% compared to the non-pegylated mIL22 (Y51A) mutant.
Species ReactivityHuman
ReconstitutionIt is recommended to reconstitute the lyophilized pegylated mouse IL22 Y51A in sterile water or sterile 0.4% NaHCO3 adjusted to pH 8-9, not less than 100µg/ml, which can then be further diluted with other aqueous solutions.
DescriptionMono-pegylated (with 20 kDa PEG)recombinant human interleukin 22 Y51A mutant (numbering according to human IL-22 including signal peptide) is one polypeptide chain containing 147 amino, an additional Ala at N-terminus and one molecule of PEG 20 kDa at its N-terminus, having an expected molecular mass of ~ 36 kDa as determined by MS. However due to enlarged hydrodymanic volume it runs on the SDS-PAGE as 50 kDa protein and in gel-filtration on Superdex 200 as over 200 kDa protein. It was prepared similarly to that described by L. Niv-Spector et al. Protein Eng Des Sel. (PEDS) 25:397-404 (2012).
Uniprot IDQ9GZX6
Protein RefSeqNP_065386.1
mRNA RefSeqNM_020525.4

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