Human EPO

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Cat-Nr.100-062
Size50 µg
Price170 €
SourceCHO cells
Formulationlyophilized
Purity Confirmation90% by SDS-PAGE & HPLC analyses
Length [aa]166
Molecular Weight18.4 kDa
Endotoxin Levels< 0.1 ng/µg of protein (<1EU/µg)
Biological ActivityDetermined by a cell proliferation assay using TF-1 cells. The expected ED50 for this effect is 0.8-1.0 ng/ml.
Species ReactivityMouse, Human
SynonymsErythropoietin, Epoetin
DescriptionErythropoietin (EPO) is a glycoprotein hormone that is principally known for its role in erythropoiesis, where it is responsible for stimulating proliferation and differentiation of erythroid progenitor cells. The differentiation of CFU-E (Colony Forming Unit-Erythroid) cells into erythrocytes can only be accomplished in the presence of EPO. Physiological levels of EPO in adult mammals are maintained primarily by the kidneys, whereas levels in fetal or neonatal mammals are maintained by the liver. EPO also can exert various non-hematopoietic activities, including vascularization and proliferation of smooth muscle, neural protection during hypoxia, and stimulation of certain B cells. EPO contains 166 amino acid residues and has a calculated molecular weight of approximately 18.4 kDa. As a result of glycosylation, Recombinant Human EPO migrates with an apparent molecular mass of 37.0 kDa by SDS-PAGE gel, under reducing and non-reducing conditions.
Protein SequenceAPPRLICDSR VLERYLLEAK EAENITTGCA EHCSLNENIT VPDTKVNFYA WKRMEVGQQA VEVWQGLALL SEAVLRGQAL LVNSSQPWEP LQLHVDKAVS GLRSLTTLLR ALGAQKEAIS PPDAASAAPL RTITADTFRK LFRVYSNFLR GKLKLYTGEA CRTGDR
Uniprot IDP01588
Protein RefSeqNP_000790.2
mRNA RefSeqNM_000799.2

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