Fish (denis) Growth Hormone

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Size50 µg
Price295 €
SourceE. coli
Purity Confirmation> 98.0% as determined by Gel filtration and SDS-PAGE.
Length [aa]188
Molecular Weight21.4 kDa
N Terminal SequenceAQPITDGQRL
Endotoxin Levels< 0.1 ng/µg of protein (<1EU/µg)
Biological ActivityBinding assays of the 125I-labeled recombinant Gilthead Seabream growth hormone to dolphin fish liver microsomal fraction resulted in high specific binding characterized by a Ka of 1.93 nM and a Bmax of 540 fmol/mg microsomal fraction protein. Recombinant Gilthead Seabream growth hormone like ovine placental lactogen, exhibited growth-stimulating activity when applied orally to S.aurata larvae or intraperitoneally to juvenile fish.
ReconstitutionIt is recommended to reconstitute the lyophilized recombinant Gilthead Seabream growth hormone in sterile 0.4% NaHCO3 adjusted, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
SynonymsSomatotropin, GH, GH-N, Growth Hormone 1, Pituitary growth hormone
DescriptionRecombinant Gilthead Seabream (Sparus aurata) growth hormone (gsGH) produced in E. coli (21.4 K) is a single, non-glycosylated, polypeptide chain containing 188 amino acids and having a molecular mass of ~ 21.392 kDa. Recombinant Gilthead Seabream growth hormone was purified by chromatographic techniques, according to Ben-Atia et al., General and Comparative Endocrinology 113, 155–164 (1999).
Uniprot IDP29971
Protein RefSeqAAB19750.2
mRNA RefSeqS54890.1

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