Rainbow trout Growth Hormone

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Size10 µg
Price190 €
SourceE. coli
Purity Confirmation> 98.0% as determined by Gel filtration and SDS-PAGE.
Length [aa]188
Molecular Weight22.0 kDa
N Terminal SequenceAIENQR
Endotoxin Levels< 0.1 ng/µg of protein (<1EU/µg)
Biological ActivityrtGH is biologically active in PDF-P1 3B9 cells stably transfected with rabbit GH receptors, though its activity is ~ 10-fold lower than that of human GH.
ReconstitutionIt is recommended to reconstitute the lyophilized rtGH in sterile water or 0.4% NaHCO3 adjusted to pH 8-9, not less than 100µg/ml and not more than 3 mg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.
SynonymsSomatotropin, GH, GH-N, Growth Hormone 1, Pituitary growth hormone
DescriptionRecombinant rainbow trout growth hormone (rtGH) produced in E. coli (22 K) is a single, non-glycosylated, polypeptide chain containing 188 amino acids with an additional Ala at its N-terminus and having a molecular mass of 21535 Dalton. rtGH is expressed as inclusion bodies, refolded and purified by proprietary chromatographic techniques.
Uniprot IDP09538
Protein RefSeqNP_001118161.1
mRNA RefSeqNM_001124689.1

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