Mouse FGF-4

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Cat-Nr.M30-130
Size5 µg
Price90 €
SourceE. coli
LabelHis-Tag
Formulationlyophilized
Purity Confirmation> 80% by SDS-PAGE & visualized by silver stain
Length [aa]195
Molecular Weight21.6 kDa
Biological ActivityThe biological activity was determined by the induction of proliferation in NHDF cells (Normal Human Dermal Fibroblasts).
Buffer0.5X PBS
ReconstitutionWe recommend a quick spin followed by reconstitution in water to a concentration of 0.1-1.0mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4 °C for 1 week or -20 °C for future use.
Stability and StorageThe lyophilized protein is stable for a few weeks at room temperature, but best stored at -20 °C. Reconstituted FGF-4 should be stored in working aliquots at -20 °C. Avoid repeated freeze-thaw cycles.
SynonymsFgf4; KS3; hst; Fgfk; Hst1; kFGF; Fgf-4; hst-1; Hstf-1
DescriptionFGF4 (fibroblast growth factor4), also known as FGF-K or K-FGF (Kaposi’s sarcoma-associated FGF), is a 25 kDa secreted, heparin-binding member of the FGF family. The mouse FGF4 cDNA encodes 202 amino acids (aa) with a 29 aa signal sequence and a 173 aa mature protein with an FGF homology domain that contains a heparin binding region near the C-terminus. Mature mouse FGF 4 shares 87%, 90%, 87% and 85% aa identity with human, rat, canine and bovine FGF4, respectively. Human FGF4 has been shown to exhibit cross species activity. Expression of FGF4 and its receptors, FGF R1c, 2c, 3c and 4, is spatially and temporally regulated during embryonic development. Its expression in the trophoblast inner cell mass promotes expression of FGF R2, and is required for maintenance of the trophectoderm and primitive endoderm. Later in development, FGF4 works together with FGF8 to mediate the activities of the apical ectodermal ridge, which direct the outgrowth and patterning of vertebrate limbs. FGF4 is proposed to play a physiologically relevant role in human embryonic stem cell self-renewal. It promotes stem cell proliferation, but may also aid differentiation depending on context and concentration, and is often included in embryonic stem cell media in vitro. A C-terminally truncated 15 kDa isoform that opposes full length FGF4 and promotes differentiation is endogenously expressed in human embryonic stem cells. FGF4 is mitogenic for fibroblasts and endothelial cells in vitro and has autocrine transforming potential. It is a potent angiogenesis promoter in vivo and has been investigated as therapy for coronary artery disease.
Protein SequenceMGHHHHHHHHHHSSGHIEGRHMAPNGTRHAELGHGWDGLVARSLARLPVAAQPPQAAVRSGAGDYLLGLKRLRRLYCNVGIGFHLQVLPDGRIGGVHADTRDSLLELSPVQRGVVSIFGVASRFFVAMSSRGKLFGVPFFTDECKFKEILLPNNYNAYEAYAYPGMFMALSKNGRTKKGNRVSPTMKVTHFLPRL
Uniprot IDP11403
Protein RefSeqNP_034332
mRNA RefSeqNM_010202

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