Human VEGF-B167

Slide this table

Cat-Nr.300-080S
Size5 µg
Price70 €
SourceE. coli
Formulationlyophilized
Purity Confirmation> 98% by SDS-PAGE & Coomassie stain
Length [aa]167
Molecular Weight38 kDa
N Terminal SequenceMPVSQ
Endotoxin Levels< 0.1 ng /mg of protein (<1EU/mg).
Biological ActivityMeasured by its binding ability in a functional ELISA. Immobilized human sVEGFR-1/Flt-1 at 1 µg/mL (100 µL/well) can bind rhVEGF-B167 with a linear range of 0.5 ng/mL to 12.5 ng/mL.
Species ReactivityHuman
Buffer50mM acetic acid
ReconstitutionCentrifuge the vial prior to opening! The lyophilized VEGF-B167 should be reconstituted in water to a concentration not lower than 50 µg/ml. For long term storage we recommend to add at least 0.1% human or bovine serum albumin.
Stability and StorageLyophilized samples are stable for greater than six months at –20°C to –70°C. Reconstituted VEGF-B167 should be stored in working aliquots at -20°C.
Synonymsvascular endothelial growth factor B; VEGFB; VRF; VEGFL
DescriptionVEGF-B, a member of the VEGF family, is a potent growth and angiogenic cytokine. It promotes DNA synthesis in endothelial cells, helps regulate angiogenesis and vascular permeability, and inhibits apoptosis in certain smooth muscle cells and neurons. VEGF-B is expressed in all tissues except the liver. It forms cell surfaced-associated disulfide linked homodimers and can form heterodimers with VEGF-A. There are two known isoforms, formed by alternative splicing, which have been designated VEGF-B167 and VEGF-B186. Both forms have identical amino-terminal sequences encoding a “cysteine knot” like structural motif, but differ in their carboxyl-terminal domains. Both VEGF-B isoforms signal only through the VEGFR1 receptor. Recombinant human VEGF-B is a 38.0 kDa disulfide-linked homodimeric protein consisting of two 167 amino acid polypeptide chains.
Protein SequenceMPVSQPDAPGHQRKVVSWIDVYTRATCQPREVVVPLTVELMGTVAKQLVPSCVTVQRCGGCCPDDGLECVPTGQHQVRMQILMIRYPSSQLGEMSLEEHSQCECRPKKKDSAVKPDSPRPLCPRCTQHHQRPDPRTCRCRCRRRSFLRCQGRGLELNPDTCRCRKLRR
Uniprot IDP49765
Protein RefSeqNP_003368.1
mRNA RefSeqNM_003377.4

All prices plus VAT + possible delivery charges