Human Osteopontin

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Cat-Nr.100-371S
Size10 µg
Price75 €
SourceHEK 293 cells
Formulationlyophilized
Purity Confirmation> 97% by SDS-PAGE & HPLC analyses
Length [aa]298
Molecular Weight60-65 kDa
Endotoxin Levels< 0.1 ng /mg of protein (<1EU/mg).
Biological ActivityDetermined by its ability to chemoattract human monocytes using a concentration range of 10.0-100.0 ng/ml.
Species ReactivityHuman
ReconstitutionCentrifuge the vial prior to opening!
SynonymsSPP1; OPN; BNSP; BSPI; ETA-1
DescriptionOsteopontin is a secreted glycoprotein that functions as a ligand to alphavbeta3 integrin and possibly other receptors. It binds tightly to hydroxyapatite and can act as a structural component of the extracellular mineralized matrix. Osteopontin is initially secreted as a 298 amino acid protein, which is subject to multiple post-translational modifications including glycosylation, phosphorylation, and specific proteolytic cleavages into various smaller molecular weight fragments. Osteopontin is expressed in a wide range of cells and tissues including osteoblasts, various tumor cell lines, and extraosseous cells in the inner ear, brain, kidney, deciduum, placenta and odontoblasts. In addition to its involvement in mineralized matrix formation, Osteopontin can also function as a cytokine that stimulates the release of IFNγ and IL-12, while inhibiting the production of IL-10. Recombinant human Osteopontin is a 298 amino acid protein, which, due to glycosylation, migrates at an apparent molecular weight of 60-65 kDa by SDS-PAGE analysis under reducing conditions.
Protein SequenceIPVKQADSGS SEEKQLYNKY PDAVATWLNP DPSQKQNLLA PQNAVSSEET NDFKQETLPS KSNESHDHMD DMDDEDDDDH VDSQDSIDSN DSDDVDDTDD SHQSDESHHS DESDELVTDF PTDLPATEVF TPVVPTVDTY DGRGDSVVYG LRSKSKKFRR PDIQYPDATD EDITSHMESE ELNGAYKAIP VAQDLNAPSD WDSRGKDSYE TSQLDDQSAE THSHKQSRLY KR
Uniprot IDP10451
Protein RefSeqNP_001035147
mRNA RefSeqNM_001040058

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