Human IL-1 beta

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Size50 µg
Price395 €
SourceE. coli
Purity Confirmation> 98% by SDS-PAGE & visualized by silver stain
Length [aa]153
Molecular Weight17.0 kDa
N Terminal SequenceAPVRSL and MAPVRS
Endotoxin Levels< 0.1ng per µg (IEU/µg) of rh IL-1ß
Biological ActivityMeasured in a cell proliferation assay using murine D10G4.1 cells. The ED50 for this effect is typically 2-10 pg/ml.
Species ReactivityHuman
ReconstitutionThe lyophilized rh IL-1ß is soluble in water and most aqueous buffers and can be reconstituted in water to a concentration of 0.1 mg/ml. This solution can be diluted into other buffered solutions or stored at -20 °C for future use.
Stability and StorageLyophilized IL-1ß although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution IL-1 ß should be stored at 4 °C between 2-7 days and for future use below -18 °C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
SynonymsIL1B; IL-1; IL1F2; IL1-BETA
DescriptionInterleukin-1 beta (IL-1beta) is produced by activated macrophages. IL-1beta stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1beta proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL1F1) and IL1 beta (IL1F2), which are the products of distinct genes. IL- 1alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. The human IL-1 beta cDNA encodes a 269 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta converting enzyme (Caspase1/ICE) to generate the active cytokine. The mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67% 78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta. Human recombinant IL-1beta produced in E. coli is a non-glycosylated,IL-1 beta polypeptide chain containing 153 amino acids and having a molecular mass of 17.0 kDa.
Uniprot IDP01584
Protein RefSeqNP_000567
mRNA RefSeqNM_000576

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