Human IL-3

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Cat-Nr.200-015
Size2 µg
Price55 €
SourceE. coli
Formulationlyophilized
Purity Confirmation>98% by SDS-PAGE
Length [aa]134
Molecular Weight15.0 kDa
N Terminal SequenceMAPMTQT
Endotoxin Levels< 0.1 ng per µg (IEU/µg) of rh IL-3
Biological ActivityThe ED50 as determined by the dose-dependent stimulation of the proliferation of TF-1 cells is <0.1 – 0.5 ng/ml. The WHO standard #91/510 was used as a control.
Species ReactivityHuman
BufferPBS
ReconstitutionThe lyophilized IL-3 should be reconstituted in water to a concentration not less than 100µg/ml. This solution can be diluted into other buffered solutions or stored at -20°C for future use.
Stability and StorageThe lyophilized IL-3, though stable at room temperature, is best stored desiccated below 0°C. Reconstituted IL-3 should be stored in working aliquots at -20°C.
SynonymsIL3; IL-3; MCGF; MULTI-CSF
DescriptionIL-3 is a hematopoietic growth factor that promotes the survival, differentiation and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages. Produced by T cells, mast cells and eosinophils, IL-3 enhances thrombopoieses, phagocytosis, and antibody-mediated cellular cytotoxicity. Its ability to activate monocytes suggests that IL-3 may have additional immunoregulatory roles. Many of the IL-3 activities depend upon co-stimulation with other cytokines. IL-3 is species-specific, variably glycosylated cytokine. Recombinant human IL-3 is a 15.0 kDa globular protein containing 134 amino acid residues.
Protein SequenceMAPMTQTTPLKTSWVNCSNMIDEIITHLKQPPLPLLDFNNLNGEDQDILMENNLRRPNLEAFNRAVKSLQNASAIESILKNLLPCLPLATAAPTRHPIHIKDGDWNEFRRKLTFYLKTLENAQAQQTTLSLAIF
Uniprot IDP08700
Protein RefSeqNP_000579
mRNA RefSeqNM_000588

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Reference

  1. Ex Vivo Expansion of Functional Human UCB-HSCs/HPCs by Coculture with AFT024-hkirre Cells. Muti ur Rehman Khan et al., Biomed Res Int. 2014; 2014: 412075.
  2. Cocultivation of umbilical cord blood CD34+ cells with retro-transduced hMSCs leads to effective amplification of long-term culture-initiating cells. Chun-Gang Xie et al., World J Gastroenterol. 2006 Jan 21; 12(3): 393–402.
  3. Cell surface sialylation and ecto-sialyltransferase activity of human CD34 progenitors from peripheral blood and bone marrow. Schwartz-Albiez R. et al., Glycoconj J. 2004;21(8-9):451-9.

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