Ovine Growth Hormone antagonist (G119R)

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Size10 µg
Price175 €
SourceE. coli
Purity Confirmation> 98.0% as determined by RP-HPLC, Gel filtration and SDS-PAGE Silver Stained gel.
Length [aa]191
Molecular Weight22.0 kDA
N Terminal SequenceATFPA
Endotoxin Levels< 0.1 ng/µg of protein (<1EU/µg)
Biological ActivityoGH G119R acts as antagonist using an in vitro bioassay in PDF-P1 3B9 cells stably transfected with rabbit GH receptors. It is capable of forming a 1:1 complex with the recombinant ovine growth hormone receptor extracellular domain (ECD) and binds to this ECD with affinity similar to the wild type oGH.
ReconstitutionIt is recommended to reconstitute the lyophilized oGH G119R in 0.4% NaHCO3 or water adjusted to pH 9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions, preferably in a presence of a carrier protein such as BSA or similar.
SynonymsSomatotropin, GH, GH-N, Growth Hormone 1, Pituitary growth hormone
DescriptionRecombinant ovine GH-22K G119R produced in E. coli is a single, non-glycosylated, polypeptide chain containing 191 amino acids and having a molecular mass of 22 kDa. It is mutated in single amino acid (G119R) according to similar bovine GH mutein described by Chen et al. (1991), Molecular Endocrinology 5:1845–1852.
Uniprot IDP67930
Protein RefSeqNP_001009315.2
mRNA RefSeqNM_001009315.3

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