Human IL-4

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Cat-Nr.200-023-DC
Size50 µg
Price290 €
SourceE. coli
Formulationlyophilized
Purity Confirmation> 98% by SDS-PAGE & visualized by silver stain
Length [aa]130
Molecular Weight14.9 kDa
N Terminal SequenceMHKCDITL
Endotoxin Levels< 0.1 ng per µg of IL-4
Biological ActivityThe ED50 as determined by the dose-dependent stimulation of human TF-1 cells is 0.1-0.5ng/ml.
Species ReactivityHuman
BufferPBS
ReconstitutionThe lyophilized IL-4 should be reconstituted in water to a concentration not less than 100µg/ml. This solution can be diluted into other buffered solutions or stored at -20 °C for future use.
Stability and StorageThe lyophilized IL-4, though stable at room temperature, is best stored desiccated below 0 °C. Reconstituted IL-4 should be stored in working aliquots at -20 °C. Avoid repeated freeze-thaw cycles.
SynonymsIL4; BSF1; IL-4; BCGF1; BSF-1; BCGF-1
DescriptionIL-4 is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. Produced by mast cells, T cells and bone marrow stromal cells, IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergy. Recombinant human IL-4 is a 14.9 kDa globular protein containing 130 amino acid residues
Protein SequenceMHKCDITLQEIIKTLNSLTEQKTLCTELTVTDIFAASKNTTEKETFCRAATVLRQFYSHHEKDTRCLGATAQQFHRHKQLIRFLKRLDRNLWGLAGLNSCPVKEANQSTLENFLERLKTIMREKYSKCSS
Uniprot IDP05112
Protein RefSeqNP_000580
mRNA RefSeqNM_000589

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