Human MesP1

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Cat-Nr.300-060
Size10 µg
Price190 €
SourceInsect cells
Formulationlyophilized
Purity Confirmation> 95% by SDS-PAGE & visualized by silver stain
Length [aa]268
Molecular Weight34 kDa
Biological ActivityTesting in progress.
Species ReactivityHuman
Buffer0.5X PBS
ReconstitutionHuman MesP1 should be reconstituted in sterile water to a concentration of 0.1 mg/ml. This solution can be diluted in water or other buffer solutions or stored at -20 °C.
Stability and StorageThe lyophilized human MesP1, though stable at room temperature, is best stored desiccated below 0 °C. Freeze/thaw cycles will result in significant loss of activity.
SynonymsMESP1; bHLHc5
DescriptionES-cell-based cardiovascular repair requires an in-depth understanding of the molecular mechanisms underlying the differentiation of cardiovascular ES cells. A candidate cardiovascular-fate inducer is the bHLH transcription factor MesP1 (1,2). As one of the earliest markers, it is expressed specifically in almost all cardiovascular precursors and is required for cardiac morphogenesis (2,3). It was shown that MesP1 is a key factor sufficient to induce the formation of ectopic heart tissue in vertebrates and increase cardiovasculogenesis by ES cells. Electrophysiological analysis showed all subtypes of cardiac ES-cell differentiation (4). MesP1 overexpression and knockdown experiments revealed a prominent function of MesP1 in a gene regulatory cascade, causing Dkk-1-mediated blockade of canonical Wnt-signalling. Independent evidence from ChIP and in vitro DNA-binding studies, expression analysis in wild-type and MesP knockout mice, and reporter assays confirm that Dkk-1 is a direct target of MesP1.
Protein SequenceMAQPLCPPLSESWMLSAAWGPTRRPPPSDKDCGRSLVSSPDSWGSTPADSPVASPARPGTLRDPRAPSVGRRGARSSRLGSGQRQSASEREKLRMRTLARALHELRRFLPPSVAPAGQSLTKIETLRLAIRYIGHLSAVLGLSEESLQRRCRQRGDAGSPRGCPLCPDDCPAQMQTRTQAEGQGQGRGLGLVSAVRAGASWGSPPACPGARAAPEPRDPPALFAEAACPEGQAMEPSPPSPLLPGDVLALLETWMPLSPLEWLPEEPK
Uniprot IDQ9BRJ9
Protein RefSeqNP_061140.1
mRNA RefSeqNM_018670.3

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