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|Purity Confirmation||>98% by SDS-PAGE|
|Molecular Weight||15.0 kDa|
|N Terminal Sequence||MAPMTQT|
|Endotoxin Levels||< 0.1 ng per µg (IEU/µg) of rh IL-3|
|Biological Activity||The ED50 as determined by the dose-dependent stimulation of the proliferation of TF-1 cells is < 0.1 – 0.5 ng/ml. The WHO standard #91/510 was used as a control.|
|Reconstitution||The lyophilized IL-3 should be reconstituted in water to a concentration not less than 100µg/ml. This solution can be diluted into other buffered solutions or stored at -20°C for future use.|
|Stability and Storage||The lyophilized IL-3, though stable at room temperature, is best stored desiccated below 0°C. Reconstituted IL-3 should be stored in working aliquots at -20°C.|
|Synonyms||IL3; IL-3; MCGF; MULTI-CSF|
|Description||IL-3 is a hematopoietic growth factor that promotes the survival, differentiation and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages. Produced by T cells, mast cells and eosinophils, IL-3 enhances thrombopoieses, phagocytosis, and antibody-mediated cellular cytotoxicity. Its ability to activate monocytes suggests that IL-3 may have additional immunoregulatory roles. Many of the IL-3 activities depend upon co-stimulation with other cytokines. IL-3 is species-specific, variably glycosylated cytokine. Recombinant human IL-3 is a 15.0 kDa globular protein containing 133 amino acid residues.|
- Sphingolipid-mediated inflammatory signaling leading to autophagy inhibition converts erythropoiesis to myelopoiesis in human hematopoietic stem/progenitor cells. M. Orsini et al., Cell Death Differ. 2019 Sep; 26(9): 1796–1812.
- Ex Vivo Expansion of Functional Human UCB-HSCs/HPCs by Coculture with AFT024-hkirre Cells. Muti ur Rehman Khan et al., Biomed Res Int. 2014; 2014: 412075.
- Cocultivation of umbilical cord blood CD34+ cells with retro-transduced hMSCs leads to effective amplification of long-term culture-initiating cells. Chun-Gang Xie et al., World J Gastroenterol. 2006 Jan 21; 12(3): 393–402.
- Cell surface sialylation and ecto-sialyltransferase activity of human CD34 progenitors from peripheral blood and bone marrow. Schwartz-Albiez R. et al., Glycoconj J. 2004;21(8-9):451-9.
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