Human Wnt-9b

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Cat-Nr.100-159
Size20 µg
Price170 €
SourceCHO cells
Formulationlyophilized
Purity Confirmation >95% by SDS-PAGE & HPLC analysis
Length [aa]335
Molecular Weight36.9 kDa
Endotoxin Levels< 0.1 ng /mg of protein (<1EU/mg).
Biological ActivityDetermined by its ability to induce alkaline phosphatase production by CCL-226 cells. 
Species ReactivityHuman
ReconstitutionCentrifuge the vial prior to opening!
SynonymsWNT15, WNT14B, UNQ6973/PRO21956
DescriptionFormerly known as Wnt-15 or Wnt-14b, Wnt-9b is a secreted glycoprotein belonging to the Wnt family of signaling proteins that are critically involved in maintaining the integrity of both embryonic and adult tissues. Wnt-9b is primarily expressed in adult kidneys and during late embryogenesis, and shares with other Wnt family members the same highly conserved lipid-modified, cysteine-rich domain essential for cell signaling. As is true for most Wnt family members, Wnt-9b functions through the biochemical process known as the canonical Wnt pathway; during which Wnt proteins bind to and activate seven-pass transmembrane receptors of the Frizzled family, and ultimately result in the disruption of beta-catenin degradation. Intracellular accumulation of beta-catenin increases translocation of the protein into the nucleus, where it binds to TCF/LEF transcription factors to promote the expression of numerous genes. In this manner, Wnt signaling induces and maintains transformed phenotype, and, in certain embryonic cell lines, supports self-renewal in the absence of significant differentiation. While increased Wnt/beta-catenin signaling is associated with tumorigenesis in a diverse set of human cancers, lack of Wnt signaling disrupts transcriptional activation of tumor suppressor genes, and has been shown to result in neoplastic transformation, oncogenesis, and human degenerative diseases. Altered Wnt-9b expression has been shown to result in the underdevelopment of the kidneys, and incomplete lip and cleft fusion in mice. Recombinant Human Wnt-9b is a monomeric glycoprotein containing 335 amino acid residues and has a calculated molecular weight of 36.9 kDa. Due to glycosylation, Recombinant Human Wnt-9b migrates at an apparent molecular weight of approximately 49-54 kDa by SDS-PAGE analysis under non-reducing conditions.
Protein SequenceSYFGLTGREV LTPFPGLGTA AAPAQGGAHL KQCDLLKLSR RQKQLCRREP GLAETLRDAA HLGLLECQFQ FRHERWNCSL EGRMGLLKRG FKETAFLYAV SSAALTHTLA RACSAGRMER CTCDDSPGLE SRQAWQWGVC GDNLKYSTKF LSNFLGSKRG NKDLRARADA HNTHVGIKAV KSGLRTTCKC HGVSGSCAVR TCWKQLSPFR ETGQVLKLRY DSAVKVSSAT NE
Uniprot IDO14905
Protein RefSeqNP_003387.1
mRNA RefSeqNM_003396.2

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