Mouse Leptin antagonist (mutant L39A/D40A/F41A)

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Cat-Nr.500-048
Size100 µg
Price210 €
SourceE. coli
Formulationlyophilized
Purity Confirmation> 98.0% as determined by Gel filtration and SDS-PAGE gel.
Length [aa]146
Molecular Weight16.0 kDa
N Terminal SequenceAVPIQ
Endotoxin Levels< 0.1 ng/µg of protein (<1EU/µg)
Biological ActivityRecombinant mouse leptin antagonist is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. It also inhibits various leptin effects in several in vitro bioassays.
Species ReactivityMouse
ReconstitutionIt is recommended to reconstitute the lyophilized recombinant mouse leptin antagonist in sterile water or sterile 0.4% NaHCO3 adjusted to pH 8-9, not less than 100µg/ml, which can then be further diluted with other aqueous solutions.
SynonymsLep; ob; obese
DescriptionRecombinant mouse leptin, one polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa, recombinant mouse leptin was mutated, resulting in L39A/D40A/F41A antagonist that was purified by proprietary chromatographic techniques. according to Salomon et al (2006) Protein Expression and PuriWcation 47, 128–136.
Protein SequenceAVPIQKVQDDTKTLIKTIVTRINDISHTQSVSAKQRVTGAAAIPGLHPILSLSKMDQTLAVYQQVLTSLPSQNVLQIANDLENLRDLLHLLAFSKSCSLPQTSGLQKPESLDGVLEASLYSTEVVALSRLQGSLQDILQQLDVSPEC
Uniprot IDP41160
Protein RefSeqNP_032519.1
mRNA RefSeqNM_008493.3

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