human E-Selectin

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Cat-Nr.100-410
Size50 µg
Price170 €
SourceCHO cells
Formulationlyophilized
Purity Confirmation >95% by SDS-PAGE & HPLC analysis
Length [aa]535
Molecular Weight58.6 kDa
Endotoxin Levels< 0.1 ng /mg of protein (<1EU/mg).
Biological ActivityMeasured by its ability to support adhesion of U937 cells, a human hystiocytic lymphoma cell line.
Species ReactivityHuman
ReconstitutionCentrifuge the vial prior to opening!
SynonymsEndothelial leukocyte adhesion molecule 1, ELAM1, CD62E, SELE
DescriptionSelectins are a family of calcium-dependent type 1 transmembrane proteins. Endothelial (E)-selectin is a heavily glycosylated transmembrane protein expressed by activated endothelial cells in microvascular linings. E-selectin, along with P-selectin and L-selectin, initiate recruitment of circulating leukocytes from blood to sites of inflammation in the vascular lining through interaction with specific cell surface associated carbohydrate determinants. E-selectin consists of an N-terminal type 1 lectin domain, an EGF-like domain, 6 sushi (CCP/SCR) domains, a transmembrane sequence, and a short cytoplasmic domain. Recombinant human E-selectin is a 58.6 kDa protein containing 535 amino acid residues, corresponding to the extracellular portion of the full length protein. Due to glycosylation, E-selectin migrates at an apparent molecular weight of approximately 65-85 kDa by SDS-PAGE analysis under reducing conditions.
Protein SequenceWSYNTSTEAM TYDEASAYCQ QRYTHLVAIQ NKEEIEYLNS ILSYSPSYYW IGIRKVNNVW VWVGTQKPLT EEAKNWAPGE PNNRQKDEDC VEIYIKREKD VGMWNDERCS KKKLALCYTA ACTNTSCSGH GECVETINNY TCKCDPGFSG LKCEQIVNCT ALESPEHGSL VCSHPLGNFS YNSSCSISCD RGYLPSSMET MQCMSSGEWS APIPACNVVE CDAVTNPANG FV
Uniprot IDP16581
Protein RefSeqNP_000441.2
mRNA RefSeqNM_000450.2

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