Human FRP-4, soluble

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Cat-Nr.S01-075
Size25 µg
Price170 €
SourceCHO cells
Formulationlyophilized
Purity Confirmation> 95% by SDS-PAGE & HPLC analyses
Length [aa]328
Molecular Weight37,8 kDa
Endotoxin Levels< 0.1 ng /mg of protein (<1EU/mg).
Biological ActivityDetermined by its ability to decrease alkaline phosphatase activity in CCL-226 cells when treated with 25ng/ml of Murine Wnt-3a.
Species ReactivityHuman
ReconstitutionCentrifuge the vial prior to opening!
SynonymsSecreted frizzled-related protein 4, DDC-4 (rat homologue)
DescriptionSecreted Frizzled-Related Proteins (sFRPs) are a family of glycosylated Wnt antagonists characterized by a conserved cysteine-rich domain that shares homology with the cysteine-rich, extracellular domain Frizzled proteins use for the binding of Wnt proteins and receptors. Lacking the transmembrane and intracellular domains of the Frizzled proteins, sFRPs function as soluble modulators of the Wnt signaling pathway through the direct binding of Wnt proteins to this cysteine-rich domain, and the resultant inhibition of Wnt receptor binding and signaling capabilities. sFRP-4 is widely distributed in a variety of embryonic and adult tissues where it can function as a circulating antiangiogenic factor, a potent proapoptotic factor, an inhibitor of insulin secretion, and a suppressor of both tumor growth and metastatic potential through disruption of the Wnt signaling pathway. Research has demonstrated the existence of a direct correlation between the downregulation and/or absence of circulating sFRP-4 and the progression of several cancer types, including ovarian, endometrial, prostate and lung. Upregulation of circulating sFRP-4 has been linked to the deterioration of glucose metabolism in the case of type 2 diabetes, as well as the suppression of the keratinocyte hyperproliferation and epidermal hyperlasia that are definitive of psoriasis. CHO cell-derived recombinant human sFRP-4 is a glycoprotein of 328 amino acid residues that contains a cysteine-rich, Frizzled-homologous domain. The calculated molecular weight of CHO cell-derived Recombinant Human sFRP-4 is 37.8 kDa; however, due to glycosylation, the protein migrates at an apparent molecular weight of approximately 55 - 65 kDa by SDS-PAGE analysis under reducing conditions.
Protein SequenceVRGAPCEAVR IPMCRHMPWN ITRMPNHLHH STQENAILAI EQYEELVDVN CSAVLRFFLC AMYAPICTLE FLHDPIKPCK SVCQRARDDC EPLMKMYNHS WPESLACDEL PVYDRGVCIS PEAIVTDLPE DVKWIDITPD MMVQERPLDV DCKRLSPDRC KCKKVKPTLA TYLSKNYSYV IHAKIKAVQR SGCNEVTTVV DVKEIFKSSS PIPRTQVPLI TNSSCQCPHI LP
Uniprot IDQ6FHJ7
Protein RefSeqNP_003005.2
mRNA RefSeqNM_003014.3

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