Human ROR2, soluble
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| Cat-Nr. | S01-074 |
| Size | 10 µg |
| Price | 370 € |
| Source | Insect cells |
| Formulation | lyophilized |
| Purity Confirmation | > 98% by SDS-PAGE & Coomassie stain |
| Length [aa] | 376 |
| Molecular Weight | 50.0 kDa |
| N Terminal Sequence | EVEVL |
| Biological Activity | Binds rh Wnt5a in a functional ELISA. |
| Species Reactivity | Human |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. The lyophilized sROR2 is soluble in water and most aqueous buffers and should be reconstituted in water to a concentration not lower than 100 µg/ml. |
| Stability and Storage | Lyophilized samples are stable for greater than six months at –20°C to –70°C. Reconstituted sROR2 should be stored in working aliquots at -70°C. |
| Synonyms | Neurotrophic tyrosine kinase, receptor-related 2 |
| Description | ROR2 is a signaling receptor for Wnt ligands that is known to play important roles in limb development, but having no essential roles known in adult tissues. Recent evidence has implicated ROR2 in mediating both canonical and non-canonical signaling pathways. ROR2 was initially found to be highly expressed in osteosarcoma and renal cell carcinomas, and has recently been found in an increasingly long list of cancers currently including melanoma, colon cancer, melanoma, squamous cell carcinoma of the head and neck, and breast cancer. In the majority of these cancer types, ROR2 expression is associated with more aggressive disease states, consistent with a role mediating Wnt signaling regardless of the canonical or non-canonical signal. Because of the pattern of tissue distribution, the association with high-risk diseases, and the cell surface localization of this receptor, ROR2 has been identified as a potential high value target for therapeutic development. However, the recent discovery that ROR2 may function through non-kinase activities challenges this strategy and opens up opportunities to target this important molecule through alternative means. |
| Protein Sequence | EVEVLDPNDP LGPLDGQDGP IPTLKGYFLN FLEPVNNITI VQGQTAILHC KVAGNPPPNV RWLKNDAPVV QEPRRIIIRK TEYGSRLRIQ DLDTTDTGYY QCVATNGMKT ITATGVLFVR LGPTHSPNHN FQDDYHEDGF CQPYRGIACA RFIGNRTIYV DSLQMQGEIE NRITAAFTMI GTSTHLSDQC SQFAIPSFCH FVFPLCDARS RTPKPRELCR DECEVLESDL CRQEYTIARS NPLILMRLQL PKCEALPMPE SPDAANCMRI GIPAERLGRY HQCYNGSGMD YRGTASTTKS GHQCQPWALQ HPHSHHLSST DFPELGGGHA YCRNPGGQME GPWCFTQNKN VRMELCDVPS CSPRDSSKMG HHHHHH |
| Uniprot ID | Q01974 |
| Protein RefSeq | NP_004551.2 |
| mRNA RefSeq | NM_004560.3 |
Figures
SDS-PAGE analysis of recombinant human sROR2 produced in insect cells. Sample was loaded in 15% SDS-polyacrylamide gel under reducing condition and stained with Coomassie blue.
Binding of recombinant human Wnt5a [Cat# 400-023] to recombinant human sROR2 [Cat# S01-074] in a functional ELISA. sROR2 was coated with 2μg/ml (100μl/well) and increasing amounts of Wnt5a was added. Detection was performed using a polyclonal rabbit anti-human Wnt5a and a goat anti-rabbit Biotin conjugated secondary antibody.
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