His-lysate
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| Cat-Nr. | LYS-001 |
| Size | 250 µg |
| Price | 99 € |
| Source | E. coli |
| Formulation | lyophilized |
| Application | WB |
| Reconstitution | Reconstitute in 250 µl SDS-gel sample buffer (1 mg/ml protein lysate), it can be used directly for sample loading and then stored at -20°C. The sample can be reused several times. |
| Description | The cell lysate was prepared from E.coli (Tuner [De3]) expressing a 14.2 kDa protein containing a C-terminal 6xHis tag. |
Figures
Western Blot Analysis of cell lysate prepared from E.coli (Tuner [De3]) expressing a 14.2 kDa protein containing a C-terminal 6xHis tag using ReliaTech's rabbit anti-His-tag antibody [Cat# 102-PA80]. As secondary antibody an AP-conjugated goat anti-rabbit antibody (Dianova) was used.
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