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Rabbit Anti-Mouse Lyve-1

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Cat-Nr.103-PA50S
Size100 µg
Price175 €
CategoryPolyclonal Antibody
Clone Nr.Rabbit IgG
Species ReactivityMouse
Formulationlyophilized
BufferPBS
ReconstitutionCentrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Stability and StorageThe lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.
PreparationProduced from sera of rabbits immunized with highly pure recombinant mouse soluble LYVE-1 produced in insect cells. The recombinant soluble LYVE-1 consists of amino acid 24 (Ala) to 228 (Gly) and is fused to a C-terminal His-tag (6xHis).
AntigenRecombinant mouse soluble Lyve-1 (RT#S01-026)
ApplicationWB, IHC (C), IF, FC
SynonymsLyve1; Xlkd1; Lyve-1; Crsbp-1; 1200012G08Rik
DescriptionLYVE-1 has been identified as a major receptor for HA (extracellular matrix glycosaminoglycan hyaluronan) on the lymph vessel wall. The deduced amino acid sequence of LYVE-1 predicts a 322-residue type I integral membrane polypeptide 41% similar to the CD44 HA receptor with a 212-residue extracellular domain containing a single Link module the prototypic HA binding domain of the Link protein superfamily. Like CD44, the LYVE-1 molecule binds both soluble and immobilized HA. However, unlike CD44, the LYVE-1 molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels. Hence, LYVE-1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels themselves.
Uniprot IDQ8BHC0
Protein RefSeqNP_444477
mRNA RefSeqNM_053247

Figures

Figure 1.
Cryo sections of mouse colon carcinoma labeled with rabbit polyclonal antibody against mouse LYVE-1 (red) [Cat# 103-PA50] and human CD31 (green). A: CD31; B: LYVE-1; C: CD31/LYVE-1
The experiments were performed by Dr. Ulrike Fiedler and Stefanie Koidel, Dept. of Vascular Biology and Angiogenesis Research Tumor Biology Center, Breisacher Str. 117, D-79106 Freiburg, Germany
Figure 2.
Immunohistochemistry with paraffin-embedded sections of mouse intestine using a polyclonal rabbit anti-mouse [Cat# 103-PA50] LYVE-1 antibody. You see the staining (red) of lymphatic endothelial cells of the intestine.
The experiments has been performed by Dr. Karsten Debel, DCS, Hamburg, Germany.
Figure 3.
Rat cardiac lymphatic microvessels, labeled with an antibody against rat Podoplanin [Cat# 104-M40] (left panel) and a antibody against mouse LYVE-1 (right panel). Image was obtained at 20x magnification on a Zeiss fluorescence microscope. Scale bar = 50 μm. The used protocol in short was: 1. Blockage of nonspecific binding; 2. Incubation with primary abs : anti-mouse Lyve1 (1:1000) / mouse anti-Podoplanin (1:400) for 60 min at RT; 3. Incubation with secondary abs: Donkey anti-rabbit Cy3 and Donkey anti-mouse FITC, 30 min at RT; 4. Mounting in DAPI-containing medium for cell nuclei labeling.
The experiments were performed by the research group of Prof. Dr. E. Brakenhielm – Rouen University (see also: Henri O et al., Circulation, March 2016).
Figure 4.
LYVE1/CD31 staining on frozen sections of the mouse prostate.
The experiments were performed by Scott Gerber & Edith Lord, PhD, University of Rochester, USA
Figure 5.
Western Analysis of anti-mouse LYVE-1. Sample was loaded in 15% SDS-polyacrylamide gel under reducing conditions.

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