Rabbit Anti-Mouse Endomucin
Slide this table
| Cat-Nr. | 103-PA49AG |
| Size | 50 µg |
| Price | 220 € |
| Category | Polyclonal Antibody |
| Clone Nr. | Rabbit IgG |
| Species Reactivity | Mouse |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots. |
| Antigen | Recombinant mouse soluble Endomucin (RT #S01-M64) |
| Application | WB, IF |
| Synonyms | Endomucin-1/2; Mucin-14 |
| Description | Endomucin (endothelial sialomucin; also Endomucin-1/2 and Mucin-14) is an 80 - 120 kDa glycoprotein member of the Endomucin family of proteins. It is expressed on endothelial cells and depending upon its glycosylation pattern, can serve as either a pro- or anti-adhesive molecule. Mouse Endomucin precursor is 261 amino acids in length. It is type I transmembrane protein that contains a 170 aa extracellular domain (ECD) (aa 21 - 190) and a 50 aa cytoplasmic region. Three splice variants exist in the ECD. One shows a deletion of aa 91 - 141, a second shows a one aa substitution for aa 91 - 129, and a third shows a one aa substitution for aa 129 - 142. Over aa 21 - 90, mouse Endomucin shares 60% and 30% aa identity with rat and human Endomucin, respectively |
| Uniprot ID | Q9ULC0 |
| Protein RefSeq | NP_001153166.1 |
| mRNA RefSeq | NM_001159694.1 |
Figures
Western Blot analysis of anti-mouse Endomucin. Sample was loaded in 15% SDS-polyacrylamide gel under reducing conditions. Lane 1: MWM (kDa); lane 2: rm sEndomucin (250ng).
FACS analysis with primary mouse endothelial cells (SnoMec).
Immunofluorescence staining (green) of cryo-sections of adult mouse kidney (fixed 15 min in 4% PFA) with anti-mouse Endomucin (5µg/ml) [Cat# 103-PA49AG] and counter staining of nuclei with Dapi.
The experiment was performed by the research group of Prof. Dr. J. Wilting and Dr. K. Buttler, University Medicine Göttingen, Germany.
All prices plus VAT + possible delivery charges