Rabbit Anti-Mouse VEGF-A
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| Cat-Nr. | 103-PA03S |
| Size | 100 µg |
| Price | 175 € |
| Category | Polyclonal Antibody |
| Clone Nr. | Rabbit IgG |
| Species Reactivity | Mouse |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots. |
| Preparation | Produced from sera of rabbits immunized with highly pure recombinant murine VEGF164. Anti-murine VEGF-A was purified by affinity chromatography with immobilized Protein A. |
| Antigen | Recombinant mouse VEGF164 (RT #M30-001) |
| Application | WB, IHC (P), E |
| Synonyms | Vegfa; Vpf; Vegf; Vegf120; Vegf164; Vegf188 |
| Description | Mouse VEGF is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, VEGF plays a prominent role in normal and pathological angiogenesis. Substantial evidence implicates VEGF in the induction of tumor metastasis and intra-ocular neovascular syndromes. VEGF signals through the three receptors; fms-like tyrosine kinase (flt-1), KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product. Recombinant murine VEGF (isoform 164) is a 39.0 kDa disulfide-linked homodimeric protein consisting of two 164 amino acid polypeptide chains. |
| Uniprot ID | Q00731 |
| Protein RefSeq | NP_001020421 |
| mRNA RefSeq | NM_001025250 |
Figures
Immunohistochemical staining of VEGF-A in paraffin-embedded mouse ventricular zone of the CNS (E17) and a perinecrotic area of a human glioblastom
The experiments were performed by Dr. Till Acker and Prof. K.H. Plate, Neurological Institute, Neuropathology, University Frankfurt, Germany
Western Blot analysis of recombinant mouse VEGF164 [Cat# M30-001], human VEGF165 [Cat# 300-036], human PlGF-1 [Cat# 300-015] and rat VEGF-C [Cat# R20-015] using a polyclonal rabbit anti-mouse VEGF-A antibody [Cat# 103-PA03]. There is a strong cross reactivity with human VEGF165 but not with human PlGF-1 and human VEGF-C.
Reference
- Erythropoietin Induces Lymph Node Lymphangiogenesis and Lymph Node Tumor Metastasis. Ae Sin Lee et al., Cancer Res. 2011 Jul 1;71(13):4506-17
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