Rabbit Anti-Human VEGFR-3/FLT-4
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| Cat-Nr. | 102-PABi22 |
| Size | 50 µg |
| Price | 220 € |
| Category | Polyclonal Antibody |
| Label | Biotin |
| Clone Nr. | Rabbit IgG |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS |
| Stabilizer/Carrier | BSA (50x), 0.02% sodium azide |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots. |
| Preparation | Produced from sera of rabbits immunized with highly pure recombinant human soluble VEGFR 3/FLT-4 (D1-D7). Anti-human VEGFR-3/FLT-4 was purified by antigen-affinity chromatography with immobilized soluble VEGFR-3/FLT-4. |
| Antigen | Recombinant human sFLT-4 (D1-7) (RT #S01-017) |
| Application | E |
| Synonyms | vascular endothelial growth factor receptor-3; FLT4; PCL; LMPH1A; VEGFR3; fms-related tyrosine kinase 4 |
| Description | Receptor tyrosine Kinase VEGFR-3, also known as FLT4, together with VEGFR1 (FIT1) and VEGFR2 (KDR/Flk-1), are the receptors for vascular endothelial growth factors (VEGF). The VEGFR family belongs to the class II subfamily of receptor tyrosine kinases (RTKs), containing a large extracellular region which is composed of seven Ig-like domains (D1–D7), a single transmembrane (TM) helix and cytoplasmic region with a tyrosine kinase activity. In VEGFR-3, the fifth Ig homology domain is proteolytically cleaved which results in polypeptides remain linked by two disulfide bonds. VEGFR-3 is widely expressed on all endothelia cells in early embryogenesis, while, in adult tissues, VEGFR-3 expression disappears from the vascular endothelial cells and is observed only on the lymphatic endothelium. VEGF-C and VEGF-D activation of VEGFR-3 plays an important role in the formation of the lymphatic vessel system. |
| Uniprot ID | P35916 |
| Protein RefSeq | NP_002011 |
| mRNA RefSeq | NM_002020 |
Figures
FLT-4 Sandwich-ELISA using recombinant human soluble FLT-4 as standard [Cat# S01-017]. Mouse anti-human FLT-4 #2E11 (Cat# 101-M37) was used as capture antibody, Biotinylated rabbit anti-human FLT-4 (Cat# 102-PABi22) was used for detection.
Rat cardiac lymphatic vessels, labeled with antibodies against human VEGFR-3/FLT4 [Cat# 102-PABi22] are revealed in green, and adjacent blood vessels, labeled with antibodies against CD31, are revealed in red. Image was obtained at 20x magnification on a Zeiss fluorescence microscope. Scale bar = 50 µm.
The used protocol in short was: 1. Blockage with NEN TNB solution (NEN TSA kit ); 2. Primary abs 1:400; 3. SA-HRP 1:800; 4. BT-FITC 1:400
The experiment was performed by the research group Inserm U1096 in Rouen, France directed by Dr Vincent Richard.
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