Rabbit Anti-Human VEGFR-2/KDR
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| Cat-Nr. | 102-PABi18 |
| Size | 50 µg |
| Price | 220 € |
| Category | Polyclonal Antibody |
| Label | Biotin |
| Clone Nr. | Rabbit IgG |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS |
| Stabilizer/Carrier | BSA (50x), 0.02% sodium azide |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots. |
| Preparation | Produced from sera of rabbits immunised with highly pure recombinant human soluble extracellular domain of KDR (110 kDa) as the immunizing antigen. Anti-human VEGFR-2/KDR was purified by antigen-affinity chromatography with immobilised recombinant soluble VEGFR-2/KDR and then biotinylated using a standard protocol. |
| Antigen | Recombinant human KDR (D1-7) (RT #S01-001) |
| Application | E |
| Synonyms | vascular endothelial growth factor receptor-2 ; KDR; FLK1; CD309; VEGF receptor 2; VEGFR2; kinase insert domain protein receptor |
| Description | VEGF R1 (Flt-1), VEGF R2 (KDR/Flk-1), and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domain and kinase insert domains in their intracellular region. They are best known for regulating VEGF family-mediated vasculogenesis, angiogenesis, and lymphangiogenesis. They are also mediators of neurotrophic activity and regulators of hematopoietic development. Human VEGF R2 is thought to be the primary inducer of VEGF-mediated blood vessel growth, while VEGF R3 plays a significant role in VEGF-C and VEGF-D-mediated lymphangiogenesis. |
| Uniprot ID | P35968 |
| Protein RefSeq | NP_002244.1 |
| mRNA RefSeq | NM_002253.2 |
Figures
VEGFR-2/KDR Sandwich-ELISA using soluble KDR (D7) [Cat# S01-002] as standard. Mouse anti-human VEGFR-2 [Cat# 101-M32] was used as capture antibody, Biotinylated rabbit anti-human VEGFR-2 [Cat# 102-PABi18] was used for detection.
Total lysate from PAE/KDR cells was measured for expression of VEGFR-1/KDR by a standard Sandwich ELISA using a mouse monoclonal anti-human VEGFR-1 antibody (#101-M32) for capturing and a Biotin conjugated rabbit polyclonal anti-human VEGFR-1 antibody (#102-PABi18) for detection. Total lysate from PAE/Flt-1 and PAE/FLT-4 was used as negative control. As positive control total lysate from PAE/Flt-1 was spiked with recombinant human sKDR (#S01-002).
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