Rabbit Anti- GFP

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Cat-Nr.102-PA84
Size50 µg
Price120 €
CategoryPolyclonal Antibody
Clone Nr.Rabbit IG
Formulationlyophilized
BufferPBS
ReconstitutionCentrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Stability and StorageThe lyophilized antibody is stable at room temperature for up to 1 month. The reconstituted antibody is stable for at least two weeks at 2-8 °C. Frozen aliquots are stable for at least 6 months when stored at -20 °C.
PreparationThe anti-GFP antibody was generated by immunization of rabbits with highly purified recombinant GFP protein produced in E. coli. The anti-GFP antibodies were purified by affinity chromatography with immobilized Protein A.
ApplicationWB
Synonymsgreen fluorescent protein
Description Green Fluorescent Protein (GFP) has quickly become a powerful research tool for assessing gene expression and subcellular protein distribution in fixed or living cells. GFP is excited by and brightly fluoresces when exposed to UV or blue light. This feature makes it ideal as a marker for use in fluorescence microscopy, cytometry, tagging fusion proteins, and assaying transcriptional regulation from gene promoters in vivo. Numerous GFP variants with enhanced and shifted emission spectra (blue, green, and yellow) have been developed through amino acid substitutions at specific residues. The antibody was made against GFP from the jellyfish Aequorea Victoria using the whole native protein as an antigen.

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