Rabbit Anti- His-tag

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Cat-Nr.102-PA80
Size50 µg
Price120 €
CategoryPolyclonal Antibody
Clone Nr.Rabbit IgG
Formulationlyophilized
BufferPBS
ReconstitutionCentrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Stability and StorageThe lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.
PreparationThe anti-His-tag antibody was generated by immunization of rabbits with different highly purified His-tagged proteins produced in insect cells. The anti-His-tag antibodies were then purified from sera by antigen-affinity chromatography using a His-peptide as matrix.
ApplicationWB
SynonymsHis-tag
DescriptionThe His-tag antibody was generated by immunization of rabbits with several recombinant His-tagged proteins produced in insect cells and E. coli. It was antigen-affinity purified using a His-tagged protein as matrix.

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Reference

  1. Lysosomal Acid Lipase Hydrolyzes Retinyl Ester and Affects Retinoid Turnover. L. Grumet et al., J Biol Chem. 2016 Aug 19; 291(34): 17977–17987.
  2. Disruption of the methyltransferase-like 23 gene METTL23 causes mild autosomal recessive intellectual disability. M. Bernkopf et al., Hum Mol Genet. 2014 Aug 1;23(15):4015-23.
  3. Adiponutrin Functions as a Nutritionally Regulated Lysophosphatidic Acid Acyltransferase. M. Kumari et al., Cell Metab. 2012 May 2;15(5): 691–702.
  4. Characterization of rat thrombin-activatable fibrinolysis inhibitor (TAFI)--a comparative study assessing the biological equivalence of rat, murine and human TAFI. K. Hillmayer et al., J Thromb Haemost. 2006 Nov;4(11):2470-7.

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