Rabbit Anti-Human CD105/Endoglin
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| Cat-Nr. | 102-PA60AG |
| Size | 50 µg |
| Price | 220 € |
| Category | Polyclonal Antibody |
| Clone Nr. | Rabbit IgG |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots. |
| Antigen | Recombinant human sCD105/Endoglin (RT #S01-025) |
| Application | WB, E, FC, IF |
| Synonyms | Endoglin; END; ORW; HHT1; ORW1; CD105 |
| Description | Endoglin, also known as CD105, is a Type I integral membrane glycoprotein with a large, disulfide-linked, extracellular region and a short, constitutively phosphorylated, cytoplasmic tail. Two splice variants of human endoglin, the S-endoglin and L-endoglin that differ in the length of their cytoplasmic tails have been identified. Endoglin is highly expressed on vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta. It is also found on activated monocytes, bone marrow pro-erythroblasts, and leukemic cells of lymphoid and myeloid lineages. Human and mouse endoglin share approximately 70% and 97 % amino acid sequence identity in their extracellular and intracellular domains, respectively. It has clearly been shown that CD105/Endoglin is required for angiogenesis and it plays a key role in heart development. Mutations in human endoglin or ALK-1 (another type I serine/threonine receptor) lead to the vascular disorder hereditary hemorrhagic telangiectasia (HHT). Mice heterozygous for endoglin have been developed as disease models for HHT. Endoglin has been shown to be a powerful marker of neovascularization. It is also useful as a functional marker that defines long-term repopulating hematopoietic stem cells. |
| Uniprot ID | P17813 |
| Protein RefSeq | NP_000109 |
| mRNA RefSeq | NM_000118 |
Figures
Western Blot analysis of recombinant human [Cat# S01-024] and mouse [Cat# S01-022] soluble CD105/Endoglin using an anti-human CD105 antibody [Cat# 102-PA60AG] directed against recombinant human soluble CD105 produced in insect cells. The SDS-PAGE was run under reducing conditions. There is a strong cross reaction with mouse CD105/Endoglin visible.
FACS analysis with primary human umbilical vein endothelial cells (HUVEC).
Immunofluorescence staining (green) of CD105/Endoglin in primary human dermal lymphatic endothelial cells (HDLEC) with anti-human CD105 (0.5µg/ml) [Cat# 102-PA60AG] and counter staining of nuclei with Dapi. As secondary antibody goat anti-rabbit ALEXA Flour 488 (Dianova) was used 1:400.
FACS analysis with primary human dermal lymphatic endothelial cells (HDLEC).
Reference
- B lymphocyte compartments in the human splenic red pulp: capillary sheaths and periarteriolar regions. B.S. Steiniger et al., Histochem Cell Biol. 2014 May;141(5):507-18.
- Elevated expression of VEGFR-3 in lymphatic endothelial cells from lymphangiomas. S. Norgall et al., BMC Cancer. 2007; 7: 105.
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