Rabbit Anti-Human MesP1
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| Cat-Nr. | 102-PA52S |
| Size | 100 µg |
| Price | 175 € |
| Category | Polyclonal Antibody |
| Clone Nr. | Rabbit IgG |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots. |
| Antigen | Recombinant human MesP1 (RT #300-060) |
| Application | WB, IHC |
| Synonyms | MESP1; bHLHc5 |
| Description | ES-cell-based cardiovascular repair requires an in-depth understanding of the molecular mechanisms underlying the differentiation of cardiovascular ES cells. A candidate cardiovascular-fate inducer is the bHLH transcription factor MesP1. As one of the earliest markers, it is expressed specifically in almost all cardiovascular precursors and is required for cardiac morphogenesis. It was shown that MesP1 is a key factor sufficient to induce the formation of ectopic heart tissue in vertebrates and increase cardiovasculogenesis by ES cells. Electrophysiological analysis showed all subtypes of cardiac ES-cell differentiation. MesP1 overexpression and knockdown experiments revealed a prominent function of MesP1 in a gene regulatory cascade, causing Dkk-1-mediated blockade of canonical Wnt-signalling. Independent evidence from ChIP and in vitro DNA-binding studies, expression analysis in wild-type and MesP knockout mice, and reporter assays confirm that Dkk-1 is a direct target of MesP1. |
| Uniprot ID | Q9BRJ9 |
| Protein RefSeq | NP_061140.1 |
| mRNA RefSeq | NM_018670 |
Figures
Western Blot analysis of anti-human MesP1. Sample was loaded in 15% SDS-polyacrylamide gel under reducing conditions.
Immunofluorescence staining of cryo-sections of unfixed human foreskin with anti-human MesP1 (dilution 1:100) and counter staining of nuclei with Dapi.
Note: The specific green MesP1 signal is visible in the epidermis and in dermal blood vessels.
The experiment was performed by the research group of Prof. Dr. J. Wilting and Dr. K. Buttler, University Medicine Göttingen, Germany.
Immunofluorescence staining of cryo-sections of unfixed human foreskin with anti-human MesP1 (dilution 1:100) and counter staining of nuclei with Dapi.
Note: The specific green MesP1 signal is visible in the epidermis and in dermal blood vessels (left and middle picture). Right picture: Negative control without primary antibody.
The experiment was performed by the research group of Prof. Dr. J. Wilting and Dr. K. Buttler, University Medicine Göttingen, Germany.
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