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Rabbit Anti-Human Prox-1

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Cat-Nr.102-PA32AG
Size50 µg
Price230 €
CategoryPolyclonal Antibody
Clone Nr.Rabbit IgG
Species ReactivityHuman
Formulationlyophilized
Buffer0.5X PBS, pH 7.2
ReconstitutionCentrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Stability and StorageThe lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.
AntigenRecombinant human Prox-1 (RT #300-052)
ApplicationWB, IF
SynonymsPROX1; W117m
DescriptionProx-1 is a homeobox gene and acts as a master switch for lymphatic endothelial phenotype. Expression of Prox-1 in blood endothelial cells induces expression of other lymphatic marker genes. Together with Podoplanin, Prox-1 can be used to reliably distinguish lymphatic vessels from blood vessels. Prox-1 is expressed in CNS, eye, pancreas, liver and heart, and it is one of the most specific and reliable markers for lymphatic endothelial cells. Produced from sera of rabbits immunized with the recombinant highly conserved C-terminal part of the homeobox transcription factor Prox-1.
Uniprot IDQ92786
Protein RefSeqNP_002754.2
mRNA RefSeqNM_002763.3

Figures

Figure 1.
Immunofluorescence staining (green) of cryo-sections of human foreskin (fixed 15 min in 4% PFA) with anti-human Prox-1 (dilution 1:200) [Cat# 102-PA32AG] and counter staining of nuclei with Dapi. A) Note the specific green Prox-1 signal in the nuclei of lymphatics (L). Ep, epidermis. Specimen provided by Prof. Dr. J. Wilting, Goettingen.
The experiment was performed by PD Dr. Jörg Wilting, Pädiatrie I, Zentrum für Kinderheilkunde und Jugendmedizin in Göttingen, Germany.
Figure 2.
Prox1 protein in chick CAM detected by immunohistochemistry studies (IHC) using a polyclonal rabbit anti-human Prox1 antibody [1μg/ml]. Prox-1 positive nuclei of lymphatic endothelial cells (LECs) are visible. (10x objective, Bar = 30μm).
The experiment was performed by the research group of Prof. Dr. J. Wilting, University Göttingen, Germany.
Figure 3.
Prox1 protein in E15 chick pancreas tissue detected by immunohistochemistry studies (IHC) using a polyclonal rabbit anti-human Prox1 antibody ( Cat# 102-PA32AG). [diluted: 1:400]
Experiment was performed by Andreia Margarido (de Santa Barbara’s team); INSERM U1046, Montpellier, France
IF Prox-1 HUVEC HDLEC
Figure 4.
Double IF stanining of human Prox-1 and CD31 in a mix of HUVEC and HDLEC with a polyclonal rabbit anti-human Prox-1 antibody [Cat# 102-PA32AG] (green) and a monoclonal mouse anti-human CD31 antibody [Cat# 101-M92] (red)
Figure 5.
Immunofluorescence staining (green) of primary human dermal lymphatic endothelial cells (HDLEC) with anti-human Prox-1 (1µg/ml) [Cat# 102-PA32AG] and counter staining of nuclei with Dapi. As secondary antibody goat anti-rabbit ALEXA Flour 488 (Dianova) was used 1:400.
Figure 6.
Prox1 protein in E15 chick pancreas tissue detected by immunofluorescence studies (IF) using a polyclonal rabbit anti-human Prox1 antibody ( Cat# 102-PA32AG). [diluted: 1:200]
Experiment was performed by Andreia Margarido (de Santa Barbara’s team); INSERM U1046, Montpellier, France
Figure 7.
Western analysis with anti-human Prox-1.

Protocol

Anti-human PROX-1

a) For IF with LECPDF download
b) For IF with LEC and HUVECPDF download

 

Reference

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